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首页> 外文期刊>Journal of bacteriology >Coliphage P1-mediated transduction of cloned DNA from Escherichia coli to Myxococcus xanthus: use for complementation and recombinational analyses.
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Coliphage P1-mediated transduction of cloned DNA from Escherichia coli to Myxococcus xanthus: use for complementation and recombinational analyses.

机译：大肠杆菌Colithage P1介导的克隆DNA从大肠杆菌向黄原粘菌的转导：用于互补和重组分析。

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摘要

We have found that coliphage P1 can be used to transduce cloned DNA from Escherichia coli to Myxococcus xanthus. Transduction occurred at a high efficiency, and no evidence for DNA restriction was observed. The analysis of the transductants showed that they fall into three general categories: (i) haploid cells which contain portions of the cloned DNA substituted for homologous chromosomal DNA; (ii) heterozygous merodiploids which contain the recombinant plasmid integrated into the chromosome at a region of homology; and (iii) homozygous merodiploids which contain two copies of a portion of the cloned DNA with the loss of the chromosomal copy of the genes. The merodiploids, once formed, are relatively stable. They were used to analyze two genes necessary for aggregation and thus fruiting body formation. P1 transduction also permits the reintroduction and substitution of mutated regions of cloned DNA into M. xanthus for the analysis of the role of the DNA in cellular physiology and development.
机译：我们已经发现，噬菌体P1可用于将克隆的DNA从大肠杆菌转导到黄色粘球菌。转导以高效率发生，并且没有观察到DNA限制的证据。对转导子的分析表明，它们分为三大类：（i）单倍体细胞，其中含有部分克隆的DNA替代了同源染色体DNA; （ii）杂合子类倍体，其包含整合到染色体同源区域的重组质粒; （iii）纯合的类金属倍体，其含有一部分克隆DNA的两个拷贝，且基因的染色体拷贝丢失。甲倍体一旦形成，就相对稳定。他们被用来分析聚集和从而形成子实体所需的两个基因。 P1转导还允许将克隆的DNA的突变区重新引入并替换为黄花木霉，以分析DNA在细胞生理学和发育中的作用。

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