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首页> 外文期刊>Journal of bacteriology >Isolation and analysis of genes involved in siderophore biosynthesis in plant-growth-stimulating Pseudomonas putida WCS358.
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Isolation and analysis of genes involved in siderophore biosynthesis in plant-growth-stimulating Pseudomonas putida WCS358.

机译：刺激植物生长假单胞菌假单胞菌WCS358中铁载体生物合成相关基因的分离和分析。

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摘要

The plant-growth-stimulating Pseudomonas putida WCS358 was mutagenized with transposon Tn5. The resulting mutant colony bank was screened for mutants defective in the biosynthesis of the fluorescent siderophore. A total of 28 mutants, divided into six different classes, were isolated that were nonfluorescent or defective in iron acquisition or both. These different types of mutants together with the probable overall structure of the siderophore, i.e., a small peptide chain attached to a fluorescing group, suggest a biosynthetic pathway in which the synthesis of the fluorescing group is preceded by the synthesis of the peptide part. A gene colony bank of P. putida WCS358 was constructed with the broad-host-range cosmid vector pLAFR1. This genomic library, established in Escherichia coli, was mobilized into the 28 individual mutants, screening for transconjugants restored in fluorescence or growth under iron-limiting conditions or both. A total of 13 cosmids were found to complement 13 distinct mutants. The complementation analysis revealed that at least five gene clusters, with a minimum of seven genes, are needed for siderophore biosynthesis. Some of these genes seem to be arranged in an operon-like structure.
机译：用转座子Tn5诱变刺激植物生长的恶臭假单胞菌WCS358。筛选所得的突变菌落库，寻找在荧光铁载体的生物合成中有缺陷的突变体。分离出总共28个突变体，分为六个不同的类别，这些突变体是非荧光性的或铁捕获缺陷或两者兼有。这些不同类型的突变体以及铁载体的可能的整体结构，即与发荧光基团连接的小肽链，提示了生物合成途径，其中发荧光基团的合成先于肽部分的合成。用宽宿主范围的粘粒载体pLAFR1构建恶臭假单胞菌WCS358的基因集落库。这个建立在大肠杆菌中的基因组文库被动员到28个个体突变体中，筛选在铁限制条件下或在铁限制条件下恢复荧光或生长的转导结合体。发现总共13个粘粒与13个不同的突变体互补。互补分析显示，铁载体生物合成至少需要五个基因簇，最少需要七个基因。这些基因中的一些似乎排列成操纵子样结构。

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