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首页> 外文期刊>Journal of bacteriology >Distribution of peptidoglycan synthetase activities between sporangia and forespores in sporulating cells of Bacillus sphaericus.
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Distribution of peptidoglycan synthetase activities between sporangia and forespores in sporulating cells of Bacillus sphaericus.

机译：球形芽孢杆菌孢子形成细胞中孢子囊和孢子之间肽聚糖合成酶活性的分布。

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Sporulating cells of Bacillus sphaericus 9602 containing fully engulfed forespores at different stages of maturity were broken by ultrasonic disruption, followed by grinding with alumina. In this way soluble enzymes derived mainly from the sporangial or from the forespore cytoplasms were obtained. Diaminopimelate ligase activity is required exclusively for cortical peptidoglycan synthesis, is absent during vegetative growth, and is synthesized during forespore maturation. It is found exclusively in the sporangial cytoplasm. L-lysine ligase is required for vegetative cell wall peptidoglycan synthesis but not for cortex synthesis. It is found in both fractions, but it has a fourfold higher specific activity in the forespore cytoplasm. Other enzymes that are required for synthesis of the nucleotide-pentapeptide precursors of both cortical and vegetative cell wall peptidoglycans are found in similar specific activities in both compartments. Mature spores, free of any residual sporangial material, have specific activities of all of these enzymes and of L-lysine ligase similar to those in forespores and in vegetative cells and are devoid of diaminopimelate ligase activity. Thus, the differential expression of at least one gene required for spore cortex synthesis in B. sphaericus occurs exclusively in the sporangial cytoplasm.
机译：通过超声破碎破坏包含在不同成熟阶段被完全吞噬的孢子的球形芽孢杆菌9602的孢子细胞，然后用氧化铝研磨。通过这种方式，获得了主要来源于孢子囊或前孢子质的可溶性酶。二氨基庚二酸酯连接酶活性仅对于皮质肽聚糖的合成是必需的，在营养生长过程中不存在，并且在前孢子成熟过程中被合成。仅在孢子囊细胞质中发现它。营养细胞壁肽聚糖合成需要L-赖氨酸连接酶，而皮质合成则不需要。在两个部分中都发现了它，但是它在前孢子细胞质中具有四倍高的比活性。皮质和营养细胞壁肽聚糖的核苷酸五肽前体合成所需的其他酶在两个区室中都以相似的比活被发现。成熟的孢子，没有任何残留的孢子囊物质，具有所有这些酶和L-赖氨酸连接酶的特异活性，类似于前孢子和营养细胞中的特异活性，并且没有二氨基庚二酸酯连接酶活性。因此，球形芽孢杆菌中至少一种孢子皮层合成所需的基因的差异表达仅在孢子囊细胞质中发生。

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