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首页> 外文期刊>Journal of bacteriology >Chromosomal and extrachromosomal deoxyribonucleic acid from four bacterial endosymbionts derived from stock 51 of Paramecium tetraurelia.
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Chromosomal and extrachromosomal deoxyribonucleic acid from four bacterial endosymbionts derived from stock 51 of Paramecium tetraurelia.

机译：来自四个脲草履虫种群51的四个细菌内共生体的染色体和染色体外脱氧核糖核酸。

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Four variant lines of stock 51 kappa (Paramecium tetraurelia) were screened for the presence of covalently closed circular (CCC) deoxyribonucleic acid (DNA). Stock 51m43 kappa, a nonkiller resistant to 51 killing, contained four classes of CCC DNA: 2.9 X 10(7), 9.7 X 10(7), and 11.8 X 10(7) daltons. The buoyant densities of 51m43 kappa chromosomal and CCC DNA were 1.700 and 1.698 g/cm3, respectively. Stock 51m43 pi, a sensitive nonkiller, contained two CCC species: 0.3 X 10(7) and 4.4 X 10(7) daltons. The buoyant densities of both the chromosomal and CCC DNA were 1.694 to 1.695 g/cm3. Three sizes of CCC DNA were found in 51m1 pi: 0.3 X 10(7), 2.3 X 10(7), and 4.5 X 10(7) daltons. The buoyant densities of both the chromosoaml DNA and the CC DNA were 1.694 to 1.695 g/cm3. It is not known whether 51m1 kappa, a sensitive spinner killer, contains CCC DNA. The buoyant density of its chromosomal DNA was 1.703 g/cm3. Of the four variant lines, only 51m43 kappa appears to be a mutant of 51 kappa. The chromosomal and CCC DNAs of 51m43 kappa have the same buoyant densities as those of 51 kappa; in addition 51m43 kappa contain a CCC molecule the same size as that found in 51 kappa (2.8 x 10(7) daltons). The three other lines are probably bacterial species that are distinct from 51 kappa and which, at one time, were co-inhabitants with 51 kappa in stock 51 paramecia.
机译：针对共价闭合的环状（CCC）脱氧核糖核酸（DNA）的存在，筛选了51种kappa（草履虫）的四个变异株系。股票51m43 kappa是一种抗51杀伤力的非杀手，包含四类CCC DNA：2.9 X 10（7），9.7 X 10（7）和11.8 X 10（7）道尔顿。 51m43 kappa染色体和CCC DNA的浮力密度分别为1.700和1.698 g / cm3。 pi 51m43 pi是一种敏感的非杀虫剂，包含两种CCC物种：0.3 X 10（7）和4.4 X 10（7）道尔顿。染色体和CCC DNA的浮力密度均为1.694至1.695 g / cm3。在51ml pi中发现了三种大小的CCC DNA：0.3 X 10（7），2.3 X 10（7）和4.5 X 10（7）道尔顿。染色体DNA和CC DNA的浮力密度为1.694至1.695g / cm 3。尚不知道51m1 kappa（一种敏感的旋转杀手）是否含有CCC DNA。其染色体DNA的浮力密度为1.703 g / cm3。在这四个变异系中，只有51m43κ突变体是51κ的突变体。 51m43 kappa的染色体和CCC DNA的浮力密度与51 kappa相同。此外，51m43 kappa包含的CCC分子大小与51 kappa中的大小相同（2.8 x 10（7）道尔顿）。另外三个系可能是不同于51 kappa的细菌种类，并且曾经与51 kappa种群的51 kappa共同居住。

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