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首页> 外文期刊>Journal of bacteriology >Electron microscope study of the rod-to-coccus shape change in a temperature-sensitive rod- mutant of Bacillus subtilis.
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Electron microscope study of the rod-to-coccus shape change in a temperature-sensitive rod- mutant of Bacillus subtilis.

机译：电子显微镜研究枯草芽孢杆菌对温度敏感的杆状突变体中杆间球菌形状的变化。

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摘要

The changes in cell morphology of Bacillus subtilis rodB during a temperature shift from 20 to 42 degrees C, in the absence of added anions, are described. At 20 degrees C the organisms grow as rods but gradually become spherical in shape when placed at 42 degrees C. The shape change is initiated by an increase in diameter at the cell equator, resulting in a bulged morphology, which is further modified to the morphology of a coccus. This change may involve a modification of the pattern of normal cylindrical extension such that incorporation of newly synthesized wall leads only to increase in diameter, perhaps from a growth zone of limited extent. The pattern of surface growth was followed by reconstructing the sequence of cross wall formation and pole construction in rods grown at 20 degrees C and in organisms incubated at 42 degrees C for 75 and 150 min. In thin section, wall forming the septum and nascent poles can be distinguished from the surface distal to the division site by the presence of raised tears, perhaps analogous to the wall bands of streptococci. By using an analog rotation technique involving the three-dimensional reconstruction of cells by mathematical rotation of axial thin sections about their longitudinal axis, it is shown that the proportion of septal wall increases during the shape change. In the coccal forms, all surface growth may arise from septal growth sites.
机译：描述了在没有添加阴离子的情况下，枯草芽孢杆菌rodB在从20到42摄氏度的温度转变过程中细胞形态的变化。在20摄氏度时，生物体以棒状生长，但在42摄氏度放置时逐渐变为球形。形状变化是由细胞赤道处直径的增大而引发的，导致凸起的形态，并进一步修饰为形态球菌。这种变化可能涉及正常圆柱状延伸图案的修改，以使新合成壁的掺入仅导致直径增加，可能来自有限程度的生长区域。表面生长的模式是通过重建在20摄氏度下生长的杆和在42摄氏度下孵育75分钟和150分钟的生物体中的横壁形成和杆构造的顺序来进行的。在薄截面中，可以通过形成凸起的眼泪（可能类似于链球菌的壁带）将形成隔膜和新生极的壁与分裂部位远端的表面区分开。通过使用模拟旋转技术，该技术包括通过轴向薄截面围绕其纵轴的数学旋转对单元进行三维重建，结果表明，在形状变化过程中，间隔壁的比例会增加。在球菌形式中，所有表面生长都可能来自间隔生长部位。

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