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首页> 外文期刊>Journal of bacteriology >Evidence that subcellular flagellin pools in Caulobacter crescentus are precursors in flagellum assembly.
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Evidence that subcellular flagellin pools in Caulobacter crescentus are precursors in flagellum assembly.

机译：有证据表明新月形杆菌中的亚细胞鞭毛蛋白池是鞭毛组装中的前体。

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To study the assembly of the Caulobacter crescentus flagellar filament, we have devised a fractionation protocol that separates the cellular flagellin into three compartments: soluble, membrane, and assembled. Radioactive labeling in pulse-chase and pulse-labeling experiments has demonstrated for the first time that both soluble and membrane-associated flagellin pools are precursors in the assembly of the flagellar filament. The results of these experiments also indicate that flagellar filament assembly occurs via the translocation of newly synthesized flagellins from the soluble pool to the membrane pool to the assembled flagellar filaments. It is not possible to conclude whether the soluble flagellin fraction is synthesized cytoplasmically or as a loosely associated membrane intermediate which is released during lysis. It is clear, however, that the soluble and membrane flagellins are in physically and functionally distinct pools. The implications of these findings for the study of protein secretion from cells and the invariant targeting of flagellar proteins to the stalk-distal pole of the dividing cell during flagellum morphogenesis are discussed.
机译：为了研究新月形杆菌鞭毛细丝的装配，我们设计了一种分馏方案，将细胞鞭毛蛋白分为三个部分：可溶，膜和已组装。脉冲追踪和脉冲标记实验中的放射性标记首次证明可溶性和膜相关鞭毛蛋白池都是鞭毛丝装配中的前体。这些实验的结果还表明，鞭毛丝的装配是通过新合成的鞭毛蛋白从可溶性库到膜池再到鞭毛丝的组装而发生的。不能断定可溶性鞭毛蛋白部分是细胞质合成的还是作为松散相关的膜中间体合成的，在裂解过程中会释放出来。但是，很明显，可溶性和膜鞭毛蛋白在物理和功能上都不同。讨论了这些发现对研究细胞分泌蛋白质以及鞭毛形态发生过程中鞭毛蛋白始终靶向分裂细胞的茎-远端极的意义。

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