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首页> 外文期刊>Journal of bacteriology >Kinetic studies on formation of cytochrome oxidase of Rhodopseudomonas capsulata after a shift from phototrophic to chemotrophic growth.
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Kinetic studies on formation of cytochrome oxidase of Rhodopseudomonas capsulata after a shift from phototrophic to chemotrophic growth.

机译：从光养生长向化养生长后，荚膜红假单胞菌细胞色素氧化酶形成的动力学研究。

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摘要

Rhodopseudomonas capsulata cells were shifted from phototrophic (anaerobic, light) to chemotrophic (semiaerobic, dark, 10% air saturation) growth conditions. During the adaptation period of 4 h, the bacteriochlorophyll content of cells and membranes decreased, and a newly synthesized 65-kilodalton polypeptide of the cytochrome oxidase was incorporated into the membrane fraction. The enzymatic activity of the cytochrome oxidase increased strongly after a lag time of 2 h. The amount of cytochrome oxidase protein does not follow the same kinetics. The relative amount of a membrane-bound cytochrome c of low molecular weight, which has been proposed to be a donor for the cytochrome oxidase, increased during adaptation.
机译：荚膜红假单胞菌细胞从光养（厌氧，光）转变为化学养护（贫血，黑暗，空气饱和度为10％）。在4 h的适应期中，细胞和膜的细菌叶绿素含量降低，并且新合成的细胞色素氧化酶的65千达尔顿多肽被掺入到膜级分中。滞后2小时后，细胞色素氧化酶的酶活性强烈增加。细胞色素氧化酶蛋白的数量并不遵循相同的动力学。已经提出是细胞色素氧化酶的供体的低分子量膜结合细胞色素c的相对量在适应过程中增加。

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