...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >Effect of sterol side chains on growth and membrane fatty acid composition of Saccharomyces cerevisiae.
【24h】

Effect of sterol side chains on growth and membrane fatty acid composition of Saccharomyces cerevisiae.

机译：固醇侧链对酿酒酵母生长和膜脂肪酸组成的影响。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Saccharomyces cerevisiae GL7 cells require exogenous sterol and unsaturated fatty acid for growth. When grown in the presence of cholesterol or 7-dehydrocholesterol, the cells incorporated less saturated fatty acid into phospholipids than cells grown with ergosterol, stigmasterol, or beta-sitosterol as the sterol source. This lower saturated fatty acid content was most pronounced in phosphatidylethanolamine, slightly less so in phosphatidylcholine, and least evident in phosphatidylserine and phosphatidylinositol. Growing the cells with the various sterols did not affect the ratios of individual phospholipids. The ability of strain GL7 to use 7-dehydrocholesterol as the only sterol supplement for growth was dependent upon the nature of the unsaturated fatty acids added to the growth medium. In the presence of linoleic, linolenic, or a mixture of palmitoleic and oleic acids, excellent growth was observed with either ergosterol, cholesterol, or 7-dehydrocholesterol. However, when the medium was supplemented with either oleic or petroselenic acid, the cells grew more slowly (oleic) or much more poorly (petroselenic) with 7-dehydrocholesterol than with ergosterol. A specific relationship between sterol structure and membrane fatty acid composition in yeast cells is implied.
机译：酿酒酵母GL7细胞需要外源固醇和不饱和脂肪酸才能生长。当在胆固醇或7-脱氢胆固醇存在下生长时，与掺入麦角固醇，豆甾醇或β-谷甾醇作为固醇源的细胞相比，该细胞将较少的饱和脂肪酸掺入磷脂中。这种较低的饱和脂肪酸含量在磷脂酰乙醇胺中最明显，在磷脂酰胆碱中则稍少，在磷脂酰丝氨酸和磷脂酰肌醇中最不明显。使细胞与各种固醇一起生长不会影响单个磷脂的比例。菌株GL7使用7-脱氢胆固醇作为唯一的固醇补充剂进行生长的能力取决于添加到生长培养基中的不饱和脂肪酸的性质。在亚油酸，亚麻酸或棕榈油酸和油酸的混合物的存在下，麦角固醇，胆固醇或7-脱氢胆固醇均观察到优异的生长。但是，当向培养基中添加油酸或石油烯酸时，与麦角固醇相比，使用7-脱氢胆固醇的细胞生长得更慢（油酸）或更差（石油醚）。暗示了酵母细胞中固醇结构和膜脂肪酸组成之间的特定关系。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号