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首页> 外文期刊>Journal of bacteriology >Complementation of argG and hisA mutations of Escherichia coli by DNA cloned from the archaebacterium Methanococcus voltae.

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Complementation of argG and hisA mutations of Escherichia coli by DNA cloned from the archaebacterium Methanococcus voltae.

机译：从伏地甲烷甲烷球菌克隆的DNA对大肠杆菌argG和hisA突变的补充。

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DNA derived from the methanogenic archaebacterium Methanococcus voltae was digested with PstI restriction endonuclease and cloned into the PstI site of pBR322. The recombinant plasmids generated were used to transform a multiply auxotrophic strain of Escherichia coli with selection for tetracycline resistance. Plasmids complementing the argG(pAW1) or hisA(pAW2) mutations were isolated and characterized. Nick-translated pAW1 and pAW2 hybridized to the predicted M. voltae PstI fragments but not to digested E. coli DNA. A novel 55,000-dalton protein was synthesized in UV-irradiated cells by pAW1, whereas pAW2 synthesized a novel 26,000-dalton protein. Derivatives of pAW1 carrying insertion elements no longer complemented the argG mutation and failed to produce the 55,000-dalton protein. When an AccI fragment was deleted from pAW2, complementation of hisA did not occur and no 26,000-dalton protein was synthesized. The effect of orientation of the cloned DNA within the vector on complementation and polypeptide synthesis was examined.

机译：用PstI限制性内切核酸酶消化源自产甲烷菌的甲烷甲烷球菌的DNA，并将其克隆到pBR322的PstI位点。产生的重组质粒用于转化大肠杆菌的多营养缺陷型菌株，并选择四环素抗性。互补argG（pAW1）或hisA（pAW2）突变的质粒被分离和鉴定。尼克翻译的pAW1和pAW2与预测的伏牛分支杆菌PstI片段杂交，但未与消化的大肠杆菌DNA杂交。 pAW1在紫外线照射的细胞中合成了一种新型的55,000道尔顿蛋白，而pAW2合成了一种新型的26,000道尔顿蛋白。带有插入元件的pAW1的衍生物不再与argG突变互补，并且无法产生55,000道尔顿的蛋白质。当从pAW2中删除AccI片段时，没有发生hisA的互补，并且没有合成26,000道尔顿的蛋白质。检查了载体内克隆的DNA的取向对互补和多肽合成的影响。

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《Journal of bacteriology》 |1983年第1期|共11页
作者
A G Wood; A H Redborg; D R Cue; W B Whitman; J Konisky;
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中图分类微生物学;
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6. Complementation of argG and hisA mutations of Escherichia coli by DNA cloned from the archaebacterium Methanococcus voltae. [O] . A G Wood, A H Redborg, D R Cue, 1983

机译：从伏地甲烷杆菌球菌中克隆的DNA对大肠杆菌argG和hisA突变的补充。
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