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首页> 外文期刊>Journal of bacteriology >L-, P-, and M-ring proteins of the flagellar basal body of Salmonella typhimurium: gene sequences and deduced protein sequences.
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L-, P-, and M-ring proteins of the flagellar basal body of Salmonella typhimurium: gene sequences and deduced protein sequences.

机译：鼠伤寒沙门氏菌鞭毛基体的L环，P环和M环蛋白质：基因序列和推导的蛋白质序列。

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The flgH, flgI, and fliF genes of Salmonella typhimurium encode the major proteins for the L, P, and M rings of the flagellar basal body. We have determined the sequences of these genes and the flgJ gene and examined the deduced amino acid sequences of their products. FlgH and FlgI, which are exported across the cell membrane to their destinations in the outer membrane and periplasmic space, respectively, both had typical N-terminal cleaved signal-peptide sequences. FlgH is predicted to have a considerable amount of beta-sheet structure, as has been noted for other outer membrane proteins. FlgI is predicted to have an even greater amount of beta-structure. FliF, as is usual for a cytoplasmic membrane protein of a procaryote, lacked a signal peptide; it is predicted to have considerable alpha-helical structure, including an N-terminal sequence that is likely to be membrane-spanning. However, it had overall a quite hydrophilic sequence with a high charge density, especially towards its C terminus. The flgJ gene, immediately adjacent to flgI and the last gene of the flgB operon, encodes a flagellar protein of unknown function whose deduced sequence was hydrophilic and may correspond to a cytoplasmic protein. Several aspects of the DNA sequence of these genes and their surrounds suggest complex regulation of the flagellar gene system. A notable example occurs within the flgB operon, where between the end of flgG (encoding the distal rod protein of the basal body) and the start of flgH (encoding the L-ring protein) there was an unusually long noncoding region containing a potential stem-loop sequence, which could attenuate termination of transcription or stabilize part of the transcript against degradation. Another example is the interface between the flgB and flgK operons, where transcription termination of the former may occur within the coding region of the latter.
机译：鼠伤寒沙门氏菌的flgH，flgI和fliF基因编码鞭毛基体L，P和M环的主要蛋白质。我们已经确定了这些基因和flgJ基因的序列，并检查了其产物的推导氨基酸序列。 FlgH和FlgI分别通过细胞膜输出到外膜和周质空间中的目的地，它们都具有典型的N端裂解信号肽序列。正如其他外膜蛋白所指出的那样，预计FlgH具有相当数量的β-sheet结构。预测FlgI具有更大数量的β-结构。 FliF，像原核生物的细胞质膜蛋白一样，缺少信号肽。据预测它具有相当大的α-螺旋结构，包括可能跨膜的N-末端序列。然而，它总体上具有相当亲水的序列，具有高电荷密度，尤其是朝向其C端。与flgI和flgB操纵子的最后一个基因紧邻的flgJ基因编码功能未知的鞭毛蛋白，其推断的序列是亲水性的，可能与细胞质蛋白相对应。这些基因及其周围环境的DNA序列的几个方面表明鞭毛基因系统的复杂调控。一个显着的例子发生在flgB操纵子中，在flgG的末端（编码基体的远端杆蛋白）和flgH的起始位置（编码L环蛋白）之间，存在一个异常长的非编码区域，其中包含潜在的茎-环序列，其可以减弱转录的终止或稳定转录物的一部分以免降解。另一个例子是flgB和flgK操纵子之间的接口，其中前者的转录终止可能发生在后者的编码区内。

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