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首页> 外文期刊>Journal of bacteriology >Isolation and characterization of the 7S RNA gene from Methanococcus voltae.
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Isolation and characterization of the 7S RNA gene from Methanococcus voltae.

机译：伏地甲烷球菌的7S RNA基因的分离和鉴定。

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摘要

The gene encoding the 7S RNA of the archaebacterium Methanococcus voltae has been isolated. The gene occurs as a single copy within the genome and encodes an RNA molecule approximately 300 nucleotides in length. The M. voltae RNA molecule exhibits a strong similarity to both archaebacterial and eucaryotic 7S RNAs in terms of overall secondary structure, while the primary sequence is conserved to a lesser degree. All 7S RNA molecules possess a specific structural domain which is highly conserved in terms of both primary sequence and secondary structure, possibly representing a functional site of the molecule. Conservation of the 7S RNA molecule suggests that it is the descendant of a subcellular structure present before the divergence of the archaebacterial and eucaryotic kingdoms. The M. voltae 7S RNA gene is flanked both 5' and 3' by regions of extremely A + T-rich DNA. The 5'-flanking region contains several potential promoter sequences for archaebacterial RNA polymerases. One such sequence occurs as three direct repeats and bears a strong similarity to sequences found upstream of other archaebacterial genes. The 3'-flanking region contains a strong signal for the termination of transcription.
机译：已经分离出编码伏地甲烷球菌的7S RNA的基因。该基因在基因组中以单拷贝形式出现，并编码一个长度约为300个核苷酸的RNA分子。就整体二级结构而言，伏特螺旋菌RNA分子与古细菌和真核7S RNA都具有很强的相似性，而一级序列的保守程度较低。所有7S RNA分子均具有特定的结构域，该结构域在一级序列和二级结构方面均高度保守，可能代表了分子的功能位点。 7S RNA分子的保守性表明它是在古细菌和真核生物王国发散之前存在的亚细胞结构的后代。伏牛分枝杆菌7S RNA基因位于5'和3'两侧，是富含A + T的非常丰富的DNA区域。 5'侧翼区包含一些古细菌RNA聚合酶的潜在启动子序列。一个这样的序列作为三个直接重复出现，并且与其他古细菌基因上游的序列具有很强的相似性。 3'侧翼区包含用于终止转录的强信号。

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