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首页> 外文期刊>Journal of bacteriology >Derepression of the synthesis of D-ribulose 1,5-bisphosphate carboxylase/oxygenase from Rhodospirillum rubrum.
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Derepression of the synthesis of D-ribulose 1,5-bisphosphate carboxylase/oxygenase from Rhodospirillum rubrum.

机译：从红螺螺旋藻中抑制D-核糖1,5-双磷酸羧化酶/加氧酶的合成。

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摘要

The synthesis of ribulose 1,5-bisphosphate carboxylase/oxygenase in Rhodospirillum rubrum was greatly influenced by the conditions of culture. When grown photolithotrophically in an atmosphere containing low levels of CO2 (1.5 to 2%), enzyme synthesis was derepressed, with the result that the enzyme comprised up to 50% of the soluble protein of the cells as determined by immunological quantitation. This response was not observed when R. rubrum was grown photolithotrophically in an atmosphere of 5% CO2 in hydrogen. Similarly, the derepression of ribulose 1,5-bisphosphate carboxylase/oxygenase was observed in photoheterotrophically (butyrate)-grown cultures only after the HCO3- supply was nearly exhausted. The increase in enzyme activity observed in derepressed cultures was not paralleled by an increase in the in vivo CO2 fixation rate. Apparently, R. rubrum derepresses the synthesis of ribulose 1,5-bisphosphate carboxylase/oxygenase when exposed to low CO2 concentrations to scavenge the limited CO2 available to such cultures.
机译：培养条件对红螺螺旋藻中核糖1，5-双磷酸羧化酶/加氧酶的合成影响很大。当在低水平的CO2（1.5％至2％）的气氛中光刻生长时，酶的合成受到抑制，结果该酶占细胞可溶性蛋白的50％（通过免疫定量分析）。当在5％CO2的氢气气氛中以光刻法生长红毛红景天时，未观察到此响应。同样，仅在几乎耗尽了HCO3的供应后，在光异养（丁酸酯）生长的培养物中观察到核糖1,5-二磷酸羧化酶/加氧酶的抑制作用。在抑制抑制的培养物中观察到的酶活性的增加与体内二氧化碳固定率的增加并没有平行。显然，当暴露于低CO 2浓度时，R。rubrum抑制了核糖1,5-双磷酸羧化酶/加氧酶的合成，以清除此类培养物中可用的有限CO2。

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