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首页> 外文期刊>Journal of bacteriology >Entry exclusion determinant(s) of IncN plasmid pKM101.
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Entry exclusion determinant(s) of IncN plasmid pKM101.

机译：IncN质粒pKM101的进入排斥决定簇。

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pKM101 renders its host a poor recipient in conjugal matings with genetically distinguishable derivatives of itself. The gene(s) primarily responsible for this, denoted eex, is located in between genes required for both conjugal transfer and sensitivity to donor-specific bacteriophage, although it itself is not necessary for transfer. A gene linked to, or coincident with, the region needed for vegetative plasmid replication also inhibited establishment of related plasmids under certain conditions. Construction of an operon fusion between eex and the Escherichia coli lac promoter has shown that this gene is transcribed in a clockwise fashion on the circular map of pKM101. To date, we have not been able to visualize a protein product(s) of the eex gene(s).
机译：pKM101使其宿主在其自身具有遗传上可区分的衍生物的配偶交配中成为不良受体。主要负责此作用的基因（称为eex）位于接合转移和对供体特异性噬菌体敏感性都需要的基因之间，尽管它本身并不是转移所必需的。与营养质粒复制所需区域相连或重合的基因在某些条件下也会抑制相关质粒的建立。 eex和大肠杆菌lac启动子之间操纵子融合体的构建表明，该基因以顺时针方式转录在pKM101的圆形图上。迄今为止，我们还无法可视化eex基因的蛋白质产物。

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