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首页> 外文期刊>Journal of bacteriology >Conjugal transfer and characterization of bacteriocin plasmids in group N (lactic acid) streptococci.
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Conjugal transfer and characterization of bacteriocin plasmids in group N (lactic acid) streptococci.

机译：N组（乳酸）链球菌中细菌素质粒的结合转移和特性鉴定。

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Thirteen bacteriocin-producing strains of group N (lactic acid) streptococci were screened for their potential to transfer this property by conjugation to Streptococcus lactis subsp. diacetylactis Bu2-60. Bacteriocin production in three strains was plasmid encoded as shown by conjugal transfer and by analysis of cured, bacteriocin-negative derivatives of the donor strains and the transconjugants. With Streptococcus cremoris strains 9B4 and 4G6 and S. lactis subsp. diacetylactis 6F7 as donors, bacteriocin-producing transconjugants were isolated with frequencies ranging from ca. 2 X 10(-2) to 2 X 10(-1) per recipient cell. Bacteriocin-producing transconjugants had acquired a 39.6-megadalton plasmid from the donor strains 9B4 and 4G6, and a 75-megadalton plasmid from the donor strain 6F7. As shown by restriction endonuclease analysis, the plasmids from strains 9B4 and 4G6 were almost identical. The plasmid from strain 6F7 yielded some additional fragments not present in the two other plasmids. In hybridization experiments any of the three plasmids strongly hybridized with each other and with some other bacteriocin but nontransmissible plasmids from other S. cremoris strains. Homology was also detected to a variety of cryptic plasmids in lactic acid streptococci.
机译：筛选了13种N组（乳酸）链球菌的产细菌素菌株，它们通过与乳链球菌亚种结合可转移该特性。二乙酰基肌动蛋白Bu2-60。如通过结合转移和通过分析供体菌株和反结合缀合物的固化的细菌素阴性衍生物显示的那样，编码了三种菌株中的细菌素生产。与Cremocooccus cremoris菌株9B4和4G6和乳酸链球菌亚种。作为供体的二乙酰基肌动蛋白6F7，分离出产生细菌素的超结合物，频率范围大约为。每个接收方细胞2 X 10（-2）至2 X 10（-1）。产生细菌素的转结合子已从供体菌株9B4和4G6中获得了39.6兆达尔顿的质粒，并从供体菌株6F7中获得了75兆达尔顿的质粒。如限制性核酸内切酶分析所示，来自菌株9B4和4G6的质粒几乎相同。来自菌株6F7的质粒产生了另外两个质粒中不存在的一些其他片段。在杂交实验中，这三个质粒中的任何一个相互之间以及与其他细菌素强烈杂交，但与其他克雷莫链霉菌菌株的非传播质粒强烈杂交。还检测到乳酸链球菌中各种隐性质粒的同源性。

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