Molecular cloning of structural and immunity genes for megacins A-216 and A-19213 in Bacillus megaterium.

M A Von Tersch; B C Carlton

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Molecular cloning of structural and immunity genes for megacins A-216 and A-19213 in Bacillus megaterium.

机译：巨大芽孢杆菌中大蛋白A-216和A-19213的结构和免疫基因的分子克隆。

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A host-vector system was developed for molecular cloning in Bacillus megaterium and used to clone the structural and immunity genes for megacins A-216 and A-19213. Recombinant clones that expressed immunity only or both immunity to and production of each megacin were obtained. Restriction mapping of native megacinogenic plasmids and recombinant clones was used to construct physical and genetic maps of megacinogenic plasmids pBM309 and pBM113. Limited sequence homology between pBM309 and pBM113 was detected by Southern blot hybridization and was mapped to, at most, a 6.4-kilobase-pair region of pBM309 and a 6.1-kilobase-pair region of pBM113.

机译：开发了一种宿主-载体系统，用于在巨大芽孢杆菌中进行分子克隆，并用于克隆大蛋白A-216和A-19213的结构和免疫基因。获得了仅表达免疫力或对每种大霉素均表达免疫力和产生表达的重组克隆。天然大基因致癌质粒和重组克隆的限制性图谱用于构建大基因致癌质粒pBM309和pBM113的物理和遗传图谱。通过Southern印迹杂交检测到pBM309和pBM113之间的有限的序列同源性，并将其定位到pBM309的6.4-碱基对区域和pBM113的6.1-碱基对区域。

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《Journal of bacteriology》 |1984年第3期|共6页
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M A Von Tersch; B C Carlton;
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1. Cloning of a new low-molecular-weight spore-specific protein gene from Bacillus megaterium. [J] . E Curiel-Quesada, P Setlow Journal of bacteriology . 1984,第3期

机译：克隆来自Megillus的新的低分子量孢子特异性蛋白基因。
2. Cloning of a new low-molecular-weight spore-specific protein gene from Bacillus megaterium. [J] . E Curiel-Quesada, P Setlow Journal of bacteriology . 1984,第3期

机译：克隆来自Megillus的新的低分子量孢子特异性蛋白基因。
3. Cloning of a new low-molecular-weight spore-specific protein gene from Bacillus megaterium. [J] . E Curiel-Quesada, P Setlow Journal of bacteriology . 1984,第3期

机译：克隆来自Megillus的新的低分子量孢子特异性蛋白基因。
4. Molecular Cloning and Biochemical Characterization of Oligo-1,6-Glucosidases from Bacillus subtilis and Bacillus licheniformis [C] . Xiaoming Hao, Ke Cai, Zixing Dong International conference on applied biotechnology . 2018

机译：枯草芽孢杆菌和地衣芽孢杆菌Oligo-1,6-葡糖苷酶的分子克隆和生化特性
5. Molecular cloning and characterization of a cluster of genes involved in the spore coat morphogenesis of Bacillus subtilis [D] . Zhang, Jianke 1993

机译：枯草芽孢杆菌芽孢形成形态相关基因簇的分子克隆与表征
6. Molecular cloning of structural and immunity genes for megacins A-216 and A-19213 in Bacillus megaterium. [O] . M A Von Tersch, B C Carlton 1984

机译：巨大芽孢杆菌中大蛋白A-216和A-19213的结构和免疫基因的分子克隆。
7. Molecular cloning of structural and immunity genes for megacins A-216 and A-19213 in Bacillus megaterium [O] . M A Von Tersch, B C Carlton 1984

机译：Megacins A-216和A-19213的结构和免疫基因的分子克隆在Bacillus MegiStium中
8. Cloning and Expression of the Bacillus anthracis Protective Antigen Gene in Bacillus subtilis. [R] . Ivins, B. E., Welkos, S. L. 1986

机译：枯草芽孢杆菌保护性抗原基因的克隆与表达。

Molecular cloning of structural and immunity genes for megacins A-216 and A-19213 in Bacillus megaterium.

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