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首页> 外文期刊>Journal of bacteriology >Level of rRNA, not tRNA, synthesis controls transcription of rRNA and tRNA operons in Escherichia coli.
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Level of rRNA, not tRNA, synthesis controls transcription of rRNA and tRNA operons in Escherichia coli.

机译:rRNA而非tRNA的合成水平控制着大肠杆菌中rRNA和tRNA操纵子的转录。

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We have recently proposed a model for the negative feedback control of rRNA and tRNA synthesis in Escherichia coli by products of rRNA operons or their derivatives (e.g., nontranslating ribosomes) (S. Jinks-Robertson, R.L. Gourse, and M. Nomura, Cell 33:865-876, 1983). In this paper, we examined the following questions. (i) Are the spacer tRNAs carried within rRNA operons the products responsible for the regulation of rRNA and tRNA transcription? (ii) Are tRNAs capable of regulating their own syntheses? We measured tRNA accumulations in cells containing plasmids with intact or defective rRNA operons or with tRNA operons. From the results obtained, we conclude that neither the tRNAs encoded within rRNA operons nor the tRNAs encoded in non-rRNA operons are capable of controlling rRNA or tRNA transcription. Therefore, the products responsible for the initial step leading to rRNA and tRNA regulation are rRNAs (or their derivatives).
机译:我们最近提出了一个模型,用于通过rRNA操纵子或其衍生物(例如,非翻译核糖体)的产物在大肠杆菌中对rRNA和tRNA合成进行负反馈控制(S. Jinks-Robertson,RL Gourse和M. Nomura,Cell 33 :865-876,1983)。在本文中,我们研究了以下问题。 (i)rRNA内携带的间隔tRNA是否负责调节rRNA和tRNA转录的产物? (ii)tRNA是否能够调节自己的合成?我们测量了含有完整或有缺陷的rRNA操纵子或tRNA操纵子的质粒的细胞中tRNA的积累。根据获得的结果,我们得出结论,rRNA操纵子内编码的tRNA或非rRNA操纵子内编码的tRNA均不能控制rRNA或tRNA转录。因此,负责导致rRNA和tRNA调控的初始步骤的产品是rRNA(或其衍生物)。

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