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首页> 外文期刊>Journal of bacteriology >Identification of genes and gene products whose expression is activated during nitrogen-limited growth in Bacillus subtilis.
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Identification of genes and gene products whose expression is activated during nitrogen-limited growth in Bacillus subtilis.

机译：鉴定在枯草芽孢杆菌中氮有限生长过程中表达被激活的基因和基因产物。

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摘要

The levels of urease and asparaginase were elevated 25- and 20-fold, respectively, in extracts of Bacillus subtilis cells grown in medium containing nitrogen sources that are poor sources of ammonium (NH4+) compared with the levels seen in extracts of cells grown in medium containing nitrogen sources that are good sources of NH4+. To determine whether a collection of genes whose expression responds to nitrogen availability could be isolated, a library of Tn917-lacZ insertions was screened for nitrogen-regulated beta-galactosidase expression. Two fusion strains were identified. beta-Galactosidase expression was 26- and 4,000-fold higher, respectively, in the nrg-21::Tn917-lacZ and the nrg-29::Tn917-lacZ insertion strains during NH4(+)-restricted growth than during growth on nitrogen sources that are good sources of NH4+. PBS1 transduction analysis showed that the nrg-21::Tn917-lacZ insertion mapped between gutB and purB and that the nrg-29::Tn917-lacZ insertion mapped between degSU and spoIID. The repression of expression of these four gene products during growth on good sources of NH4+ required the wild-type glutamine synthetase protein but not the glutamine synthetase regulatory protein, GlnR.
机译：与在培养基中生长的细胞提取物中所见的水平相比，在含有氮源（铵离子（NH4 +）来源较弱）的培养基中生长的枯草芽孢杆菌细胞提取物中，脲酶和天冬酰胺酶的水平分别升高了25倍和20倍。所含氮源是NH4 +的良好来源。为了确定是否可以分离表达对氮的有效性作出反应的基因集合，针对氮调节的β-半乳糖苷酶表达，筛选了Tn917-lacZ插入文库。确定了两种融合菌株。在NH4（+）限制生长期间，nrg-21 :: Tn917-lacZ和nrg-29 :: Tn917-lacZ插入菌株中的β-半乳糖苷酶表达分别比在氮素生长期间高26-和4,000-倍NH4 +的良好来源。 PBS1转导分析表明，nrg-21 :: Tn917-lacZ插入位于gutB和purB之间，而nrg-29 :: Tn917-lacZ插入位于degSU和spoIID之间。在良好的NH4 +来源上生长期间抑制这四个基因产物的表达需要野生型谷氨酰胺合成酶蛋白，而不需要谷氨酰胺合成酶调节蛋白GlnR。

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