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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Identification of a gene, spoIIR, that links the activation of sigma E to the transcriptional activity of sigma F during sporulation in Bacillus subtilis.
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Identification of a gene, spoIIR, that links the activation of sigma E to the transcriptional activity of sigma F during sporulation in Bacillus subtilis.

机译：在枯草芽孢杆菌的孢子形成过程中，将sigmaIIR的激活与sigma F的转录活性联系起来的基因spoIIR的鉴定。

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摘要

Sporulation of Bacillus subtilis requires the coordinated expression of two separate developmental programs in the mother cell and forespore compartments by sigma E and sigma F, respectively. This coordination is maintained through the action of cross-regulatory factors that control the activities of the various sporulation-specific sigma factors. We present here the isolation and characterization of one such cross-regulatory factor, the spoIIR gene. Using a genetic screen, we have isolated four mutant alleles of spoIIR. These mutants were isolated as expressing sigma F-directed genes but not sigma E-directed genes. The block in sigma E-directed gene expression in spoIIR mutants was caused by an inability to process pro-sigma E to its active form. Cloning and characterization of the spoIIR gene determined that its transcription is directed by sigma F. Thus, SpoIIR is required for linking the activation of sigma E to the activation of sigma F and coordinating the initiation of the two developmental programs required to form a spore.
机译：枯草芽孢杆菌的孢子形成需要分别通过sigma E和sigma F在母细胞和前孢子区室中协同表达两个独立的发育程序。通过控制各种孢子特异性西格玛因子活动的交叉调节因子的作用，可以维持这种协调。在这里，我们介绍了一种这样的交叉调节因子spoIIR基因的分离和鉴定。使用遗传筛选，我们分离了spoIIR的四个突变等位基因。这些突变体被分离为表达sigma F指导基因，而不是sigma E指导基因。 spoIIR突变体中sigma E定向基因表达受阻是由于无法将pro-sigma E加工成其活性形式引起的。 spoIIR基因的克隆和鉴定确定其转录受sigma F指导。因此，需要SpoIIR将sigma E的激活与sigma F的激活联系起来，并协调形成孢子所需的两个发育程序的启动。

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