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首页> 外文期刊>Journal of bacteriology >Detergent (sodium dodecyl sulfate) shock proteins in Escherichia coli.
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Detergent (sodium dodecyl sulfate) shock proteins in Escherichia coli.

机译:大肠杆菌中的去污剂(十二烷基硫酸钠)休克蛋白。

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摘要

The protein composition of Escherichia coli W3110 grown in the presence and absence of 5% sodium dodecyl sulfate (SDS) was examined by two-dimensional gel electrophoresis. In SDS-grown cells, at least 4 proteins were turned on, 13 were turned off, 15 were elevated, and 15 were depressed. The 19 unique and elevated SDS-induced spots constituted 7.91% of the total 35S-labeled protein. There was no apparent overlap between these 19 detergent (SDS) stress proteins and those of other known bacterial stress responses. The detergent stress stimulon is a distinct and independent stimulon. Its physiological relevance probably derives from the presence of bile salts in animal gastrointestinal tracts.
机译:通过二维凝胶电泳检查在存在和不存在5%十二烷基硫酸钠(SDS)的情况下生长的大肠杆菌W3110的蛋白质组成。在SDS生长的细胞中,至少打开4种蛋白质,关闭13种蛋白质,升高15种蛋白质,抑制15种蛋白质。 19个独特且升高的SDS诱导斑点占35S标记的总蛋白的7.91%。这19种去污剂(SDS)应激蛋白与其他已知细菌应激反应的蛋白之间没有明显的重叠。去污剂应激刺激物是独特且独立的刺激物。它的生理相关性可能源于动物胃肠道中胆盐的存在。

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