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首页> 外文期刊>Journal of bacteriology >An integrated approach to studying regulation of production of the antibiotic methylenomycin by Streptomyces coelicolor A3(2).
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An integrated approach to studying regulation of production of the antibiotic methylenomycin by Streptomyces coelicolor A3(2).

机译：研究链霉菌A3（2）调节抗生素甲基霉素的生产的综合方法。

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摘要

A physiological and molecular biological study was made of the control of methylenomycin biosynthesis by Streptomyces coelicolor A3(2). A simple and reliable assay for this antibiotic was developed. Conditions that permit the synthesis of methylenomycin by S. coelicolor cultures grown in defined medium were elucidated: a readily assimilated carbon and nitrogen source is required. Under these conditions methylenomycin is produced late in the growth phase, at the time of transition from exponential to linear growth. Provided that the phosphate concentration in the medium is kept high, there is synthesis of methylenomycin but not of the other secondary metabolites that this strain can produce. These conditions were used to study the transcription of the methylenomycin gene cluster during the transition from primary to secondary metabolism. The biosynthetic genes of at least one of the mmy transcription units appear to be transcribed before the mmr resistance determinant. The possibility that methylenomycin induces the transcription of mmr is discussed.
机译：进行了生理和分子生物学研究，以控制天蓝色链霉菌A3（2）控制甲基霉素的生物合成。开发了一种简单可靠的抗生素检测方法。阐明了允许在特定培养基中生长的天蓝色链霉菌培养物合成甲基霉素的条件：需要容易吸收的碳和氮源。在这些条件下，甲基壮霉素在生长期的后期生长，即从指数型向线性增长过渡时。只要保持培养基中的磷酸盐浓度高，就可以合成甲基霉素，但不能合成该菌株可以产生的其他次级代谢产物。这些条件用于研究从初级代谢到次级代谢过程中甲基霉素的基因簇的转录。至少一个mmy转录单位的生物合成基因似乎在mmr抗性决定簇之前被转录。讨论了甲基埃诺霉素诱导mmr转录的可能性。

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