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首页> 外文期刊>Journal of bacteriology >Organization and nucleotide sequences of the genes encoding the biotin carboxyl carrier protein and biotin carboxylase protein of Pseudomonas aeruginosa acetyl coenzyme A carboxylase.
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Organization and nucleotide sequences of the genes encoding the biotin carboxyl carrier protein and biotin carboxylase protein of Pseudomonas aeruginosa acetyl coenzyme A carboxylase.

机译：铜绿假单胞菌乙酰辅酶A羧化酶的生物素羧基载体蛋白和生物素羧化酶蛋白的编码基因的组织和核苷酸序列。

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摘要

The genetic organization of the Pseudomonas aeruginosa acetyl coenzyme A carboxylase (ACC) was investigated by cloning and characterizing a P. aeruginosa DNA fragment that complements an Escherichia coli strain with a conditional lethal mutation affecting the biotin carboxyl carrier protein (BCCP) subunit of ACC. DNA sequencing and RNA blot hybridization studies indicated that the P. aeruginosa accB (fabE) homolog, which encodes BCCP, is part of a 2-gene operon that includes accC (fabG), the structural gene for the biotin carboxylase subunit of ACC. P. aeruginosa homologs of the E. coli accA and accD, encoding the alpha and beta subunits of the ACC carboxyltransferase, were identified by hybridization of P. aeruginosa genomic DNA with the E. coli accA and accD. Data are presented which suggest that P. aeruginosa accA and accD homologs are not located either immediately upstream or downstream of the P. aeruginosa accBC operon. In contrast to E. coli, where BCCP is the only biotinylated protein, P. aeruginosa was found to contain at least three biotinylated proteins.
机译：铜绿假单胞菌乙酰辅酶A羧化酶（ACC）的遗传组织是通过克隆和鉴定铜绿假单胞菌DNA片段来研究的，该片段与大肠杆菌菌株互补，并带有条件致死性突变，影响ACC的生物素羧基载体蛋白（BCCP）亚基。 DNA测序和RNA印迹杂交研究表明，编码BCCP的铜绿假单胞菌accB（fabE）同源物是2基因操纵子的一部分，该操纵子包括accC（fabG），这是ACC生物素羧化酶亚基的结构基因。通过将铜绿假单胞菌基因组DNA与大肠杆菌accA和accD杂交来鉴定大肠杆菌accA和accD的铜绿假单胞菌同源物，编码ACC羧基转移酶的α和β亚基。提供的数据表明铜绿假单胞菌accA和accD同源物并不位于铜绿假单胞菌accBC操纵子的紧邻上游或下游。与BCCP是唯一的生物素化蛋白的大肠杆菌相反，发现铜绿假单胞菌含有至少三种生物素化蛋白。

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