Purification and properties of two membrane alkaline phosphatases from Bacillus subtilis 168.

T Sugahara; Y Konno; H Ohta; K Ito; J Kaneko; Y Kamio; K Izaki

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Purification and properties of two membrane alkaline phosphatases from Bacillus subtilis 168.

机译：枯草芽孢杆菌168中两种膜碱性磷酸酶的纯化和性质。

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Two alkaline phosphatases were extracted from the membranes of Bacillus subtilis 168 stationary-phase cells and purified as homogeneous proteins by hydroxyapatite column chromatography. Alkaline phosphatases I and II differed in several properties such as subunit molecular weight, substrate specificity, thermostability, Km, pH stability, and peptide maps.

机译：从枯草芽孢杆菌168固定相细胞膜中提取了两种碱性磷酸酶，并通过羟磷灰石柱色谱纯化为均质蛋白。碱性磷酸酶I和II在几个特性方面有所不同，例如亚基分子量，底物特异性，热稳定性，Km，pH稳定性和肽图。

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《Journal of bacteriology》 |1991年第5期|共3页
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T Sugahara; Y Konno; H Ohta; K Ito; J Kaneko; Y Kamio; K Izaki;
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中图分类微生物学;
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1. Use of alkaline phosphatase as a reporter polypeptide to study the role of the subtilin leader segment and the SpaT transporter in the posttranslational modifications and secretion of subtilin in Bacillus subtilis 168. [J] . G Izaguirre, J N Hansen Applied and Environmental Microbiology . 1997,第10期

机译：碱性磷酸酶作为报告多肽的用途，用于研究枯草杆菌前导片段和SpaT转运蛋白在枯草芽孢杆菌168中翻译后修饰和枯草杆菌蛋白酶分泌中的作用。
2. Sporulation in Bacillus subtilis 168. Comparison of alkaline phosphatase from sporulating and vegetative cells [J] . A. R. Glenn, J. Mandelstam The biochemical journal . 1971,第2期

机译：枯草芽孢杆菌168的孢子形成。孢子和营养细胞中碱性磷酸酶的比较
3. Purification and characterization of extracellular soluble and membrane-bound insoluble alkaline phosphatases possessing phosphodiesterase activities in Bacillus subtilis. [J] . K Yamane, B Maruo Journal of bacteriology . 1978,第1期

机译：枯草芽孢杆菌中具有磷酸二酯酶活性的细胞外可溶性和膜结合不溶性碱性磷酸酶的纯化和表征。
4. Purification of Alkaline Pectinase in Engineering Bacillus subtilis [C] . Ming Li, Xin Sun, Liying Zhou, International conference on applied biotechnology . 2014

机译：枯草芽孢杆菌中碱性果胶酶的纯化
5. Cloning and purification of the two component system GraSR involved in glycopeptide antibiotic resistance in Staphylococcus aureus (Mu50), and characterization of the citrate/methylcitrate synthase from Bacillus subtilis strain 168. [D] . Onyemachi, Jeremiah. 2011

机译：克隆和纯化金黄色葡萄球菌（Mu50）中涉及糖肽抗生素耐药性的两组分系统GraSR，并表征了枯草芽孢杆菌菌株168的柠檬酸/甲基柠檬酸合酶。
6. Purification and properties of two membrane alkaline phosphatases from Bacillus subtilis 168. [O] . T Sugahara, Y Konno, H Ohta, 1991

机译：枯草芽孢杆菌168中两种膜碱性磷酸酶的纯化和性质。
7. Purification and properties of two membrane alkaline phosphatases from Bacillus subtilis 168. [O] . Sugahara, T, Konno, Y, Ohta, H, 1991

机译：枯草芽孢杆菌168中两种膜碱性磷酸酶的纯化和性质。

Purification and properties of two membrane alkaline phosphatases from Bacillus subtilis 168.

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