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首页> 外文期刊>Journal of bacteriology >Roles of FliK and FlhB in determination of flagellar hook length in Salmonella typhimurium.
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Roles of FliK and FlhB in determination of flagellar hook length in Salmonella typhimurium.

机译：FliK和FlhB在确定鼠伤寒沙门氏菌鞭毛钩长中的作用。

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摘要

The length of flagellar hooks isolated from wild-type and mutant cells with various hook lengths were measured on electron micrographs. The length of the wild-type hook showed a narrow distribution with a peak (+/- standard deviation) at 55.0 +/- 5.9 nm, whereas fliK mutants (so-called polyhook mutants) showed a broad distribution of hook lengths ranging from 40 to 900 nm, strongly indicating that FliK is involved in hook length determination. Among pseudorevertants isolated from such polyhook mutants, fliK intragenic suppressors gave rise to polyhook filaments. However, intergenic suppressors mapping to flhB also gave rise to hooks of abnormal length, albeit they were much shorter than polyhooks. Furthermore, double mutations of flhB and flgK (the structural gene for hook-associated protein 1; HAP1) resulted in polyhooks, suggesting another way in which hook length can be affected. The roles of FliK, FlhB, and HAP1 in hook length determination are discussed.
机译：在电子显微照片上测量了从具有各种钩长度的野生型和突变细胞中分离出的鞭毛钩的长度。野生型钩的长度显示出狭窄的分布，在55.0 +/- 5.9 nm处有一个峰（+/-标准偏差），而fliK突变体（所谓的多钩突变体）显示出钩子长度的广泛分布，范围为40到900nm，强烈表明FliK参与钩长度的确定。从此类多钩突变体分离出的假回复株中，fliK基因内抑制子产生了多钩丝。但是，映射到flhB的基因间抑制子也引起异常长度的钩，尽管它们比多钩短得多。此外，flhB和flgK（钩相关蛋白1的结构基因; HAP1）的双重突变导致多钩，这表明可以影响钩长度的另一种方法。讨论了FliK，FlhB和HAP1在挂钩长度确定中的作用。

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