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首页> 外文期刊>Journal of bacteriology >Catabolite repression of the Bacillus subtilis hut operon requires a cis-acting site located downstream of the transcription initiation site.
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Catabolite repression of the Bacillus subtilis hut operon requires a cis-acting site located downstream of the transcription initiation site.

机译：枯草芽孢杆菌小屋操纵子的分解代谢阻遏需要一个顺式作用位点位于转录起始位点的下游。

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摘要

Expression of the Bacillus subtilis hut operon is subject to regulation by catabolite repression. A set of hut-lacZ transcriptional fusions was constructed and used to identify two cis-acting sites involved in catabolite repression. The hutOCR1 operator site lies immediately downstream of the hut promoter and weakly regulates hut expression in response to catabolite repression. The downstream hutOCR2 operator site lies within the hutP gene, between positions +203 and +216, and is required for wild-type levels of catabolite repression. Both the hutOCR1 and hutOCR2 operators have sequence similarity to the sites which mediate catabolite repression of several other B. subtilis genes. Two mutations which relieve catabolite repression of hut expression were found to alter the nucleotide sequence of the hutOCR2 operator. Catabolite repression of hut expression was partially relieved in strains containing the ccpA mutation but not in strains containing either the pai or hpr mutation.
机译：枯草芽孢杆菌小屋操纵子的表达受到分解代谢物阻抑的调控。构建了一套hut-lacZ转录融合体，并用于鉴定参与分解代谢物阻遏的两个顺式作用位点。 hutOCR1操纵位点紧靠小屋启动子的下游，并响应分解代谢物的抑制而弱调节小屋的表达。下游的hutOCR2操纵位点位于hutP基因内，在+203和+216位之间，是野生型水平的分解代谢物阻遏所必需的。 hutOCR1和hutOCR2操纵子都与介导其他几个枯草芽孢杆菌基因的分解代谢物阻遏位点具有序列相似性。发现了两个缓解小屋表达的分解代谢物阻遏的突变，它们改变了hutOCR2操纵子的核苷酸序列。在含有ccpA突变的菌株中，小屋表达的分解代谢抑制得到部分缓解，但在含有pai或hpr突变的菌株中则没有。

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