Purification of the LysR family regulator, ClcR, and its interaction with the Pseudomonas putida clcABD chlorocatechol operon promoter.

W M Coco; M R Parsek; A M Chakrabarty

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Purification of the LysR family regulator, ClcR, and its interaction with the Pseudomonas putida clcABD chlorocatechol operon promoter.

机译：LysR家族调节剂ClcR的纯化及其与恶臭假单胞菌clcABD氯邻苯二酚操纵子启动子的相互作用。

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Previous studies have shown that the clcABD operon is under the transcriptional control of the LysR-type activator ClcR. In this study, the conditions leading to its aggregation were avoided and ClcR was purified and confirmed by amino-terminal sequencing. Gel filtration indicated that ClcR exists as a dimer in solution. The DNase I footprint of ClcR was determined. The binding properties of ClcR and the catechol operon regulator, CatR, were compared.

机译：先前的研究表明，clcABD操纵子在LysR型激活子ClcR的转录控制之下。在这项研究中，避免了导致其聚集的条件，并且纯化了ClcR，并通过氨基末端测序对其进行了确认。凝胶过滤表明ClcR在溶液中以二聚体形式存在。确定了ClcR的DNase I足迹。比较了ClcR和邻苯二酚操纵子调节剂CatR的结合特性。

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《Journal of bacteriology》 |1994年第17期|共4页
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W M Coco; M R Parsek; A M Chakrabarty;
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1. Purification of the LysR family regulator, ClcR, and its interaction with the Pseudomonas putida clcABD chlorocatechol operon promoter. [J] . W M Coco, M R Parsek, A M Chakrabarty Journal of bacteriology . 1994,第17期

机译：LysR家族调节剂ClcR的纯化及其与恶臭假单胞菌clcABD氯邻苯二酚操纵子启动子的相互作用。
2. Nucleotide sequence and initial functional characterization of the clcR gene encoding a LysR family activator of the clcABD chlorocatechol operon in Pseudomonas putida. [J] . W M Coco, R K Rothmel, S Henikoff, Journal of bacteriology . 1993,第2期

机译：恶臭假单胞菌中编码clcABD氯邻苯二酚操纵子的LysR家族激活子的clcR基因的核苷酸序列和初始功能表征。
3. Nucleotide sequencing and characterization of Pseudomonas putida catR: a positive regulator of the catBC operon is a member of the LysR family. [J] . R K Rothmel, T L Aldrich, J E Houghton, Journal of bacteriology . 1990,第2期

机译：恶臭假单胞菌catR的核苷酸测序和表征：catBC操纵子的阳性调节子是LysR家族的成员。
4. A Pseudomonas putida IclR-Type Transcriptional Regulator Is Involved in Rhizosphere Related Interactions [D] . Qin, Sophie 2018

机译：恶臭假单胞菌IclR型转录调节剂参与根际相关的相互作用。
5. Purification of the LysR family regulator ClcR and its interaction with the Pseudomonas putida clcABD chlorocatechol operon promoter. [O] . W M Coco, M R Parsek, A M Chakrabarty 1994

机译：LysR家族调节剂ClcR的纯化及其与恶臭假单胞菌clcABD氯邻苯二酚操纵子启动子的相互作用。
6. Purification of the LysR family regulator, ClcR, and its interaction with the Pseudomonas putida clcABD chlorocatechol operon promoter. [O] . Coco, W M, Parsek, M R, Chakrabarty, A M 1994

机译：LysR家族调节剂ClcR的纯化及其与恶臭假单胞菌clcABD氯邻苯二酚操纵子启动子的相互作用。

Purification of the LysR family regulator, ClcR, and its interaction with the Pseudomonas putida clcABD chlorocatechol operon promoter.

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