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首页> 外文期刊>Journal of bacteriology >Intergeneric transfer of deoxyribonucleic acid killer plasmids, pGKl1 and pGKl2, from Kluyveromyces lactis into Saccharomyces cerevisiae by cell fusion.
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Intergeneric transfer of deoxyribonucleic acid killer plasmids, pGKl1 and pGKl2, from Kluyveromyces lactis into Saccharomyces cerevisiae by cell fusion.

机译：通过细胞融合将脱氧核糖核酸杀伤质粒pGKl1和pGKl2从乳酸克鲁维酵母转移到酿酒酵母中。

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Two novel linear deoxyribonucleic acid plasmids, pGKl1 and pGKl2, were isolated from the yeast Kluyveromyces lactis. K. lactis strains harboring the pGK1 plasmids killed a certain group of yeasts, including Saccharomyces cerevisiae, Saccharomyces italicus, Saccharomyces rouxii, K. lactis, Kluyveromyces thermotolerans, Kluyvermyces vanudenii, Torulopsis glabrata, Candida utilis, and Candida intermedia. In this experiment, the pGKl1 and pGKl2 plasmids were intergenerically transferred from a K. lactis killer strain into a non-killer (killer-sensitive) strain of S. cerevisiae by the use of a protoplast fusion technique. Both of the pGKl plasmids replicated autonomously and stably in the new host cells of S. cerevisiae and could coexist with the resident 2-micrometers deoxyribonucleic acid plasmid. The S. cerevisiae cells which accepted the pGKl plasmids expressed the same killer phenotype as that of the donor K. lactis killer and became resistant to the K. lactis killer. The pGKl plasmids existing in the S. cerevisiae cells were cured by treatment with ethidium bromide, and the killer and resistance characters were simultaneously lost. From there results, it was concluded that both the killer and the resistance genes are located on the pGKl plasmids.
机译：从乳酸克鲁维酵母中分离出两个新颖的线性脱氧核糖核酸质粒pGKl1和pGKl2。带有pGK1质粒的乳酸克鲁维酵母菌株杀死了某些酵母，包括酿酒酵母，酿酒酵母，鲁氏酵母，乳酸克鲁维酵母，嗜热克鲁维酵母，克鲁维酵母，凡氏假单胞菌，毛状假单胞菌，假丝酵母​​和假丝酵母。在该实验中，通过使用原生质体融合技术将pGKl1和pGKl2质粒从乳酸克鲁维酵母杀手菌株代际转移到酿酒酵母的非杀手（杀手敏感）菌株中。这两种pGK1质粒都在啤酒酵母的新宿主细胞中自主稳定地复制，并且可以与驻留的2-微米脱氧核糖核酸质粒共存。接受pGK1质粒的酿酒酵母细胞表达与供体乳酸克鲁维酵母杀手相同的杀伤表型，并对乳酸克鲁维酵母杀手产生抗性。存在于酿酒酵母细胞中的pGK1质粒通过用溴化乙锭处理而治愈，并且同时丧失了杀手和抗性特征。从那里的结果可以得出结论，杀伤基因和抗性基因都位于pGK1质粒上。

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