Isolation of Saccharomyces cerevisiae TRP3.

J L Paluh; H Zalkin

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Isolation of Saccharomyces cerevisiae TRP3.

机译：酿酒酵母TRP3的分离。

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Several plasmids, isolated from two plasmid pools, complemented a Saccharomyces cerevisiae trp3 mutant with defective indole-3-glycerol-phosphate synthase activity. Restriction mapping indicated that a 1.2-kilobase StuI segment was common to all complementing plasmids. Southern blot hybridization established that a cloned 5.2-kilobase BamHI fragment was derived intact from chromosomal DNA. A yeast trp3 mutant transformed with trp3-complementing plasmids contained approximately 40-fold elevated indole-3-glycerol-phosphate synthase activity. These plasmids also complemented an Escherichia coli trpC mutant, and transformants exhibited enzyme activity. Yeast trp3 is therefore associated with a 1.2-kilobase StuI DNA segment.

机译：从两个质粒库中分离出的几个质粒与具有缺陷的吲哚-3-甘油-磷酸合酶活性的酿酒酵母trp3突变体互补。限制性酶切图谱表明，所有互补质粒均具有1.2个碱基的StuI区段。 Southern印迹杂交确定，克隆的5.2碱基碱基的BamHI片段是完整自染色体DNA衍生的。用补全trp3的质粒转化的酵母trp3突变体含有大约40倍的吲哚-3-甘油-磷酸合酶活性。这些质粒还补充了大肠杆菌trpC突变体，并且转化体表现出酶活性。因此，酵母trp3与1.2碱基碱基的StuI DNA片段相关。

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《Journal of bacteriology》 |1983年第1期|共5页
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J L Paluh; H Zalkin;
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中图分类微生物学;
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Isolation of Saccharomyces cerevisiae TRP3.

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