Proteins induced by anaerobiosis in Escherichia coli.

M W Smith; F C Neidhardt

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Proteins induced by anaerobiosis in Escherichia coli.

机译：大肠杆菌中厌氧菌诱导的蛋白质。

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The contribution of protein induction and repression to the adaptation of cells to changes in oxygen supply is only poorly understood. We assessed this contribution by measuring the levels of 170 individual polypeptides produced by Escherichia coli K-12 in cells growing aerobically or anaerobically with and without nitrate. Eighteen reached their highest levels during anaerobic growth. These 18 polypeptides include at least 4 glycolytic enzymes and pyruvate formate-lyase (beta-subunit). Most of these proteins were found at significant levels during aerobic growth and appeared to undergo metabolic regulation by stimuli other than anaerobiosis. Anaerobic induction ratios ranged from 1.8- to 11-fold, and nitrate antagonized the anaerobic induction of all of the proteins except one. The time course of synthesis of the proteins after shifts in oxygen supply revealed at least three distinct temporal patterns. These results are discussed in light of known physiological alterations associated with changes in oxygen availability.

机译：蛋白质诱导和抑制对细胞适应氧供应变化的贡献知之甚少。我们通过测量在有或没有硝酸盐的有氧或无氧条件下生长的细胞中大肠杆菌K-12产生的170种个体多肽的水平来评估这种贡献。在厌氧菌生长期间，有18个菌落达到最高水平。这18种多肽包括至少4种糖酵解酶和丙酮酸甲酸盐裂解酶（β亚基）。这些蛋白质中的大多数在有氧生长过程中被发现处于显着水平，并且似乎是通过厌氧菌以外的刺激进行代谢调节的。厌氧诱导比范围为1.8到11倍，硝酸盐拮抗除一种蛋白质以外的所有蛋白质的厌氧诱导。氧气供应变化后蛋白质合成的时间过程揭示了至少三个不同的时间模式。根据已知的与氧利用度变化相关的生理变化讨论了这些结果。

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《Journal of bacteriology》 |1983年第1期|共8页
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M W Smith; F C Neidhardt;
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6. Proteins induced by anaerobiosis in Escherichia coli. [O] . M W Smith, F C Neidhardt 1983

机译：大肠杆菌中厌氧菌诱导的蛋白质。
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机译：诱导型调节蛋白用于识别调控子的用途：应用于大肠杆菌中亮氨酸反应性调控蛋白（Lrp）调控的调控子。
8. Characterization of Virulence Plasmids and Plasmid-Associated Outer Membrane Proteins in Shigella flexneri, Shigella sonnei, and Escherichia coli. [R] . Hale, T. L., Sansonetti, P. J., Schad, P. A., 1983

机译：弗氏志贺氏菌，志贺氏志贺菌和大肠杆菌中毒力质粒和质粒相关外膜蛋白的表征。

Proteins induced by anaerobiosis in Escherichia coli.

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