Enzymatic activity of precursors of Bacillus megaterium spore protease.

R H Hackett; P Setlow

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Enzymatic activity of precursors of Bacillus megaterium spore protease.

机译：巨大芽孢杆菌孢子蛋白酶前体的酶活性。

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The protease that initiates rapid proteolysis during germination of Bacillus megaterium spores is synthesized during sporulation as a 46,000-molecular-weight polypeptide (P46) and is processed later in sporulation to a 41,000-molecular-weight polypeptide (P41), which is converted to a 40,000-molecular-weight polypeptide (P40) early in spore germination. P40 is known to be both tetrameric and enzymatically active. In this work, we show that P46 and P41 are both tetrameric, but that only P41 is enzymatically active. The identification of a zymogen form (P46) of this protease explains in part the regulation of the activity of this enzyme.

机译：在巨大芽孢杆菌芽孢萌发过程中启动快速蛋白水解的蛋白酶在孢子形成过程中合成为46,000分子量的多肽（P46），随后在孢子形成过程中加工成41,000分子量的多肽（P41），然后将其转化为孢子萌发初期的40,000分子量多肽（P40）。已知P40具有四聚体和酶活性。在这项工作中，我们表明P46和P41都是四聚体，但是只有P41具有酶活性。该蛋白酶的酶原形式（P46）的鉴定部分解释了该酶活性的调节。

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《Journal of bacteriology》 |1983年第1期|共4页
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R H Hackett; P Setlow;
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中图分类微生物学;
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1. Integration and mapping of Bacillus megaterium genes which code for small, acid-soluble spore proteins and their protease. [J] . M D Sussman, P S Vary, C Hartman, Journal of bacteriology . 1988,第10期

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2. Isolation and characterization of Bacillus megaterium mutants containing decreased levels of spore protease. [J] . C J Postemsky, S S Dignam, P Setlow Journal of bacteriology . 1978,第3期

机译：含有降低水平的孢子蛋白酶的巨大芽孢杆菌突变体的分离和鉴定。
3. Antibacterial and Sporicidal Activities of Methanolic Sygygium polyanthum L. Leaf Extract against Vegetative Cells and Spores of Bacillus pumilus and Bacillus megaterium [J] . A.M. Abdelgani, Y. Rukayadi, K. Shaari, Journal of Pure & Applied Microbiology . 2018,第3期

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5. EFFECTS OF ULTRAVIOLET AND VISIBLE RADIATION ON THE INSECTICIDAL ACTIVITY OF THE SPORES AND CRYSTALS OF BACILLUS THURINGIENSIS (PIERIS BRASSICAE). [D] . MITCHELL, JUDITH K. 1990

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6. Enzymatic activity of precursors of Bacillus megaterium spore protease. [O] . R H Hackett, P Setlow 1983

机译：巨大芽孢杆菌孢子蛋白酶前体的酶活性。
7. Enzymatic activity of precursors of Bacillus megaterium spore protease [O] . R H Hackett, P Setlow 1983

机译：芽孢杆菌孢子蛋白酶前体的酶活性

Enzymatic activity of precursors of Bacillus megaterium spore protease.

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