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首页> 外文期刊>Journal of bacteriology >Reorienting and expanding the physical map of temperate Bacillus subtilis bacteriophage phi 105.
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Reorienting and expanding the physical map of temperate Bacillus subtilis bacteriophage phi 105.

机译：重新定向和扩大温带枯草芽孢杆菌噬菌体phi 105的物理图谱。

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摘要

During the course of extending the physical map of Bacillus subtilis temperate bacteriophage phi 105 to include SstI, XhoI, and HpaI, we recognized that the previous physical map for EcoRI was incorrect. The new enzyme maps were determined by single, double, and partial enzyme digestions, redigestion of purified phage fragments, end joint analysis, and DNA-DNA hybridization. The EcoRI physical map was corrected by double digestion of isolated fragments, DNA hybridization, and physical mapping by partial digestion of end-labeled fragments. EcoRI fragment G was repositioned to give the order D-G-I-E-B-H-F-C.
机译：在将枯草芽孢杆菌温带噬菌体phi 105的物理图谱扩展到包括SstI，XhoI和HpaI的过程中，我们认识到先前的EcoRI物理图谱是不正确的。新的酶图谱可通过单，双和部分酶消化，重新纯化的噬菌体片段，末端接头分析和DNA-DNA杂交来确定。 EcoRI物理图谱通过分离片段的双重消化，DNA杂交和末端标记片段的部分消化的物理作图得到校正。将EcoRI片段G重新定位以给出顺序D-G-I-E-B-H-F-C。

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