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首页> 外文期刊>Journal of bacteriology >Cloning and characterization of a pair of novel genes that regulate production of extracellular enzymes in Bacillus subtilis.
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Cloning and characterization of a pair of novel genes that regulate production of extracellular enzymes in Bacillus subtilis.

机译：调节枯草芽孢杆菌中胞外酶产生的一对新基因的克隆和鉴定。

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摘要

Two novel Bacillus subtilis genes that regulate the production of several extracellular enzymes were clones and characterized. These two genes are organized as part of an operon. When cloned in a multicopy plasmid, the first gene (tenA, transcription enhancement) stimulates alkaline protease production at the transcriptional level. The second gene (tenI) exerts an opposite effect to reduce alkaline protease production. The production of neutral protease, levansucrase, and alkaline protease can be stimulated up to 11- to 55-fold. Thus, tenA is a new member of the deg (regulatory genes for degradative enzymes) family in B. subtilis. A functional degS product is required to observe the stimulatory effect from tenA. Between the promoter and the ribosome-binding site of tenA, there exists a terminatorlike structure. Deletion of this structure doubles the expression of tenA. Neither tenA nor tenI is essential for cell growth and the production of extracellular enzymes. However, inactivation of these genes causes a delay in sporulation. This operon is located close to tre on the genetic linkage map. The overall organization of this operon and its relationship with other known regulatory factors in the deg family are discussed.
机译：克隆和表征了两个新的枯草芽孢杆菌基因，它们调节几种细胞外酶的产生。这两个基因是操纵子的一部分。当克隆到多拷贝质粒中时，第一个基因（tenA，转录增强）会在转录水平刺激碱性蛋白酶的产生。第二个基因（tenI）发挥相反的作用，以减少碱性蛋白酶的产生。中性蛋白酶，左旋蔗糖酶和碱性蛋白酶的产生最多可刺激11至55倍。因此，tenA是枯草芽孢杆菌中deg（降解酶的调控基因）家族的新成员。需要功能性degS产物才能观察到tenA的刺激作用。在tenA的启动子和核糖体结合位点之间，存在着类似终止子的结构。该结构的删除使tenA的表达加倍。 tenA和tenI都不是细胞生长和细胞外酶生产所必需的。但是，这些基因的失活导致孢子形成的延迟。该操纵子位于遗传连锁图谱上靠近tre的位置。讨论了该操纵子的整体结构及其与deg家族中其他已知调节因子的关系。

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