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首页> 外文期刊>Journal of bacteriology >Loci of Mycobacterium avium ser2 gene cluster and their functions.
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Loci of Mycobacterium avium ser2 gene cluster and their functions.

机译：鸟分枝杆菌ser2基因簇的基因座及其功能。

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The highly antigenic glycopeptidolipids present on the surface of members of the Mycobacterium avium complex serve to distinguish these bacteria from all others and to define the various serovars that compose this complex. Previously, the genes responsible for the biosynthesis of the disaccharide hapten [2,3-di-O-methyl-alpha-L-fucopyranosyl-(1-->3)-alpha-L-rhamnopyranose] of serovar 2 of the M. avium complex were isolated, localized to a contiguous 22- to 27-kb fragment of the M. avium genome, and designated the ser2 gene cluster (J. T. Belisle, L. Pascopella, J. M. Inamine, P. J. Brennan, and W. R. Jacobs, Jr., J. Bacteriol. 173:6991-6997, 1991). In the present study, transposon saturation mutagenesis was used to map the specific genetic loci within the ser2 gene cluster required for expression of this disaccharide. Four essential loci, termed ser2A, -B, -C, and -D, constituting a total of 5.7 kb within the ser2 gene cluster, were defined. The ser2B and ser2D loci encode the methyltransferases required to methylate the fucose at the 3 and 2 positions, respectively. The rhamnosyltransferase was encoded by ser2A, whereas either ser2C or ser2D encoded the fucosyltransferase. The ser2C and ser2D loci are also apparently involved in the de novo synthesis of fucose. Isolation of the truncated versions of the hapten induced by the transposon insertions provides genetic evidence that the glycopeptidolipids of M. avium serovar 2 are synthesized by an initial transfer of the rhamnose unit to the peptide core followed by fucose and finally O methylation of the fucosyl unit.
机译：存在于鸟分枝杆菌复合物成员表面上的高抗原性糖肽脂可将这些细菌与所有其他细菌区分开，并定义组成该复合物的各种血清型。以前，负责M.血清型2的二糖半抗原[2,3-di-O-甲基-α-L-呋喃葡糖基-（1-> 3）-α-L-鼠李吡喃糖]生物合成的基因。分离鸟复合体，定位于鸟笼分枝杆菌基因组的22-27kb连续片段，并命名为ser2基因簇（JT Belisle，L.Pascopella，JM Inamine，PJ Brennan和WR Jacobs，Jr. J.Bacteriol.173：6991-6997，1991）。在本研究中，转座子饱和诱变被用于定位表达该二糖所需的ser2基因簇内的特定遗传基因座。定义了四个必需的基因座，分别称为ser2A，-B，-C和-D，构成ser2基因簇内的总共5.7 kb。 ser2B和ser2D位点分别编码在3和2位甲基化岩藻糖所需的甲基转移酶。鼠李糖基转移酶由鼠李糖基转移酶编码，而ser2C或ser2D则编码岩藻糖基转移酶。 ser2C和ser2D基因座显然也参与了岩藻糖的从头合成。转座子插入诱导的半抗原截短形式的分离提供了遗传学证据，即鼠李糖分枝杆菌血清型2的糖肽脂质是通过鼠李糖单元最初转移到肽核心，然后是岩藻糖，最后是岩藻糖基单元的O甲基化合成。

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