Identification of sequences necessary for transcription in vitro from the Chlamydia trachomatis rRNA P1 promoter.

M Tan; J N Engel

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Identification of sequences necessary for transcription in vitro from the Chlamydia trachomatis rRNA P1 promoter.

机译：鉴定沙眼衣原体rRNA P1启动子进行体外转录所需的序列。

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Chlamydia trachomatis RNA polymerase was partially purified by heparin-agarose chromatography and used in conjunction with a plasmid-borne G-less cassette template to characterize the C. trachomatis rRNA P1 promoter in vitro. Stepwise mutational analysis revealed that sequences in the -10, -25, and -35 regions are necessary for promoter activity, but no sequence upstream of position -40 is required. Partially purified C. trachomatis RNA polymerase and purified Escherichia coli holoenzyme exhibited some differences in promoter specificity.

机译：沙眼衣原体RNA聚合酶通过肝素-琼脂糖色谱法部分纯化，并与质粒携带的无G盒模板结合使用，以体外表征沙眼衣原体rRNA P1启动子。逐步突变分析表明，-10，-25和-35区的序列对于启动子活性是必需的，但在-40位上游不需要序列。部分纯化的沙眼衣原体RNA聚合酶和纯化的大肠杆菌全酶在启动子特异性上显示出一些差异。

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《Journal of bacteriology》 |1996年第23期|共8页
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M Tan; J N Engel;
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中图分类微生物学;
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专利

1. Mutational Analysis of the Chlamydia trachomatis rRNA P1 Promoter Defines Four Regions Important for Transcription In Vitro [J] . Ming Tan, Tamas Gaal, Richard L. Gourse, Journal of bacteriology . 1998,第9期

机译：沙眼衣原体rRNA P1启动子的突变分析确定了四个重要的体外转录区域
2. Reliability of detecting rRNA sequences of Chlamydia trachomatis with fluorescence in situ hybridization without amplification. [J] . Kapur S, Abmed M, Singh V, Acta Cytologica: The Journal of Clinical Cytology and Cytopathology . 2006,第3期

机译：通过荧光原位杂交无需扩增即可检测沙眼衣原体rRNA序列的可靠性。
3. Mutations in 23S rRNA and ribosomal protein L4 account for resistance in Chlamydia trachomatis strains selected in vitro by macrolide passage. [J] . Zhu H, Wang HP, Jiang Y, Andrologia . 2010,第4期

机译：23S rRNA和核糖体蛋白L4的突变是沙眼衣原体通过大环内酯通过体外选择的菌株的抗性。
4. Application of s-LASCA technique for processing of GB-speckles, coding sequences of nucleotide sequences of omp1 gene of Chlamydia trachomatis [C] . O. V. Ulianova, Yu. V. Saltykov, S. S. Zaytsev, International Symposium on Optics and Biophotonics: Saratov Fall Meeting . 2019

机译：S-Lasca技术在加工GB斑点的应用，Chlamydia Trachomatis OMP1基因核苷酸序列编码序列
5. In vitro susceptibility of Chlamydia trachomatis to LPS-binding polyamines and cellulose ether polymers: towards the development of a microbicide against Chlamydia infection. [D] . Osaka, Ichie. 2013

机译：沙眼衣原体对LPS结合多胺和纤维素醚聚合物的体外敏感性：趋向于开发一种抗衣原体感染的杀菌剂。
6. Identification of sequences necessary for transcription in vitro from the Chlamydia trachomatis rRNA P1 promoter. [O] . M Tan, J N Engel 1996

机译：鉴定沙眼衣原体rRNA P1启动子进行体外转录所需的序列。
7. Identification of sequences necessary for transcription in vitro from the Chlamydia trachomatis rRNA P1 promoter. [O] . Tan, M, Engel, J N 1996

机译：鉴定沙眼衣原体rRNA P1启动子进行体外转录所需的序列。

Identification of sequences necessary for transcription in vitro from the Chlamydia trachomatis rRNA P1 promoter.

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