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首页> 外文期刊>Journal of bacteriology >Characterization of the gene for a protein kinase which phosphorylates the sporulation-regulatory proteins Spo0A and Spo0F of Bacillus subtilis.
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Characterization of the gene for a protein kinase which phosphorylates the sporulation-regulatory proteins Spo0A and Spo0F of Bacillus subtilis.

机译：磷酸化枯草芽孢杆菌孢子调节蛋白Spo0A和Spo0F的蛋白激酶基因的表征。

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摘要

The kinA (spoIIJ) locus contains a single gene which codes for a protein of 69,170 daltons showing strong homology to the transmitter kinases of two component regulatory systems. The purified kinase autophosphorylates in the presence of ATP and mediates the transfer of phosphate to the Spo0A and Spo0F sporulation regulatory proteins. Spo0F protein was a much better phosphoreceptor for this kinase than Spo0A protein in vitro. Mutants with deletion mutations in the kinA gene were delayed in their sporulation. They produced about a third as many spores as the wild type in 24 h, but after 72 h on solid medium, the level of spores approximated that found for the wild-type strain. Such mutations had no effect on the regulation of the abrB gene or on the timing of subtilisin expression and therefore did not impair the repression function of the Spo0A protein. Placement of the kinA locus on a multicopy vector suppressed the sporulation-defective phenotype of spo0B, spo0E, and spo0F mutations but not of spo0A mutations. The results suggest that the spo0B-, spo0E-, and spo0F-dependent pathway of activation (phosphorylation) of the Spo0A regulator may be by-passed through the kinA gene product if it is present at sufficiently high intracellular concentration. The results suggest that multiple kinases exist for the Spo0A protein.
机译：kinA（spoIIJ）基因座包含一个单一基因，该基因编码69,170道尔顿的蛋白质，与两个组分调控系统的递质激酶具有很强的同源性。纯化的激酶在ATP存在下自磷酸化，并介导磷酸盐向Spo0A和Spo0F孢子形成调节蛋白的转移。与体外Spo0A蛋白相比，Spo0F蛋白对该激酶具有更好的磷受体。 kinA基因中具有缺失突变的突变体的孢子形成被延迟。他们在24小时内产生的孢子约是野生型的三分之一，但在固体培养基上72小时后，孢子的水平接近于野生型菌株。此类突变对abrB基因的调节或枯草杆菌蛋白酶表达的时间没有影响，因此不会削弱Spo0A蛋白的阻遏功能。 kinA基因座在多拷贝载体上的放置抑制了spo0B，spo0E和spo0F突变的孢子缺陷表型，但没有抑制spo0A突变的孢子缺陷表型。结果表明，如果以足够高的细胞内浓度存在kinA基因产物，则可以绕过kinA基因产物绕过spo0B-，spo0E-和spo0F依赖的激活（磷酸化）途径。结果表明，Spo0A蛋白存在多种激酶。

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