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首页> 外文期刊>Journal of bacteriology >Identification and characterization of genes controlled by the sporulation-regulatory gene spo0H in Bacillus subtilis.
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Identification and characterization of genes controlled by the sporulation-regulatory gene spo0H in Bacillus subtilis.

机译：枯草芽孢杆菌中由孢子调节基因spo0H控制的基因的鉴定和表征。

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摘要

We describe a general strategy for the identification of genes that are controlled by a specific regulatory factor in vivo and the use of this strategy to identify genes in Bacillus subtilis that are controlled by spo0H, a regulatory gene required for the initiation of sporulation. The general strategy makes use of a cloned regulatory gene fused to an inducible promoter to control expression of the regulatory gene and random gene fusions to a reporter gene to monitor expression in the presence and absence of the regulatory gene product. spo0H encodes a sigma factor of RNA polymerase, sigma H, and is required for the extensive reprograming of gene expression during the transition from growth to stationary phase and during the initiation of sporulation. We identified 18 genes that are controlled by sigma H (csh genes) in vivo by monitoring expression of random gene fusions to lacZ, made by insertion mutagenesis with the transposon Tn917lac, in the presence and absence of sigma H. These genes had lower levels of expression in the absence of sigma H than in the presence of sigma H. Patterns of expression of the csh genes during growth and sporulation in wild-type and spo0H mutant cells indicated that other regulatory factors are probably involved in controlling expression of some of these genes. Three of the csh::Tn917lac insertion mutations caused noticeable phenotypes. One caused a defect in vegetative growth, but only in combination with a spo0H mutation. Two others caused a partial defect in sporulation. One of these also caused a defect in the development of genetic competence. Detailed characterization of some of the csh genes and their regulatory regions should help define the role of spo0H in the regulation of gene expression during the transition from growth to stationary phase and during the initiation of sporulation.
机译：我们描述了一种在体内识别受特定调节因子控制的基因的一般策略，并使用该策略来鉴定枯草芽孢杆菌中受spo0H（启动孢子形成所需的调节基因）控制的基因。一般策略是利用与诱导型启动子融合的克隆调控基因来控制调控基因的表达，以及与报告基因的随机基因融合来监测在调控基因产物存在与否下的表达。 spo0H编码RNA聚合酶的一个sigma因子sigma H，在从生长到静止期的过渡以及孢子形成的起始过程中，基因表达的大量重编程是必需的。我们通过监测在存在和不存在sigma H的情况下通过与转座子Tn917lac进行插入诱变而形成的lacZ随机基因融合体的表达，确定了18个受sigma H（csh基因）控制的体内基因。这些基因的水平较低在没有sigma H的情况下表达比在有sigma H的情况下表达。野生型和spo0H突变细胞在生长和孢子形成过程中csh基因的表达模式表明，其他调控因子可能参与了其中一些基因的表达控制。 csh :: Tn917lac插入突变中的三个引起明显的表型。一种引起营养生长缺陷，但仅与spo0H突变结合。另外两个造成部分孢子形成缺陷。其中之一也导致遗传能力发展的缺陷。一些csh基因及其调控区的详细表征应有助于确定spo0H在从生长到静止期的转变以及孢子形成的起始过程中在基因表达调控中的作用。

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