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首页> 外文期刊>Journal of bacteriology >The pcnB gene of Escherichia coli, which is required for ColE1 copy number maintenance, is dispensable.
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The pcnB gene of Escherichia coli, which is required for ColE1 copy number maintenance, is dispensable.

机译：维持ColE1拷贝数所需的大肠杆菌pcnB基因是可有可无的。

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摘要

The pcnB gene product of Escherchia coli is required for copy number maintenance of plasmids related to ColE1 and also for that of the IncFII plasmid R1. Because PcnB is similar to the tRNA-binding protein tRNA nucleotidyltransferase, we have suggested that the protein would be required only for processes in which an RNA is a prominent regulatory component. This appears to be so; strains deleted for pcnB, although defective in ColE1 and R1 plasmid maintenance, maintain the iteron-regulated plasmids F and P1 normally. We also find that strains deleted for pcnB grow normally, demonstrating that PcnB has no essential cellular role under the conditions tested and suggesting that regulation by antisense RNAs similar to RNAI has no critical role in any essential host process. We confirm by immunological tests that PcnB is likely to be the commercially available enzyme poly(A) polymerase.
机译：大肠杆菌的pcnB基因产物是维持与ColE1相关的质粒的拷贝数以及IncFII质粒R1的拷贝数所必需的。因为PcnB与tRNA结合蛋白tRNA核苷酸转移酶相似，所以我们建议只有在RNA是重要的调控成分的过程中才需要该蛋白。看来是这样;尽管在ColE1和R1质粒的维持方面存在缺陷，但缺失了pcnB的菌株，却能正常维持受iteron调控的质粒F和P1。我们还发现缺失pcnB的菌株正常生长，表明PcnB在所测试的条件下没有必需的细胞作用，这表明类似于RNAI的反义RNA的调控在任何必需的宿主过程中都没有关键作用。我们通过免疫学测试证实PcnB可能是市售的酶poly（A）聚合酶。

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