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首页> 外文期刊>Journal of bacteriology >Effect of alteration of charged residues at the N termini of signal peptides on protein export in Bacillus subtilis.
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Effect of alteration of charged residues at the N termini of signal peptides on protein export in Bacillus subtilis.

机译：信号肽N末端带电残基的改变对枯草芽孢杆菌蛋白质输出的影响。

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摘要

The role of positively charged residues at the N termini of signal peptides in protein export has been studied in Bacillus subtilis. Bacillus signal peptides (alkaline protease [Apr] and neutral protease [Npr] from Bacillus amyloliquefaciens) were altered and fused to mature levansucrase (Lvs). The effects of the various alterations on the export of Lvs in B. subtilis were determined. The replacement of positively charged residues with neutral residues in both Apr and Npr signal peptides resulted in a slight defect in the export of Lvs from B. subtilis. Introduction of a negatively charged residue (aspartic acid) at the N terminus of Npr signal peptide blocked the export of Lvs. However, Apr signal peptide with a net charge of -3 (three aspartic acid residues) was still functional.
机译：在枯草芽孢杆菌中已经研究了信号肽N末端带正电荷的残基在蛋白质输出中的作用。改变了芽孢杆菌信号肽（来自解淀粉芽孢杆菌的碱性蛋白酶[Apr]和中性蛋白酶[Npr]）并融合至成熟的葡糖蔗糖酶（Lvs）。确定了各种变化对枯草芽孢杆菌Lvs出口的影响。在Apr和Npr信号肽中用中性残基取代带正电荷的残基导致枯草芽孢杆菌Lvs的出口略有缺陷。在Npr信号肽的N末端引入带负电荷的残基（天冬氨酸）可阻止Lvs的输出。然而，具有-3的净电荷（三个天冬氨酸残基）的Apr信号肽仍然起作用。

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