Cloning, sequencing, and expression in Escherichia coli of the gene encoding a 45-kilodalton protein, elongation factor Tu, from Chlamydia trachomatis serovar F.

Y X Zhang; Y Shi; M Zhou; G A Petsko

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Cloning, sequencing, and expression in Escherichia coli of the gene encoding a 45-kilodalton protein, elongation factor Tu, from Chlamydia trachomatis serovar F.

机译：沙眼衣原体沙眼衣原体F编码45公斤蛋白，延伸因子Tu的基因的克隆，测序和在大肠杆菌中的表达。

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The gene encoding a 45-kDa protein (45K) of Chlamydia trachomatis serovar F was cloned, sequenced, and overexpressed in Escherichia coli. Alignment of the deduced peptide sequence with E. coli elongation factor Tu (EF-Tu) demonstrated 69% identity. The 45K was recognized by a Chlamydia genus-specific monoclonal antibody GP-45 and cross-reacted with a monospecific polyclonal antibody to E. coli EF-Tu. Purified recombinant 45K has the capability to bind GDP, and the binding was enhanced in the presence of E. coli elongation factor Ts (EF-Ts). The GDP binding was specifically inhibited by the monoclonal antibody GP-45. These data suggest that the 45K is a chlamydial EF-Tu, and it forms a functional complex with E. coli EF-Ts protein.

机译：克隆，编码并在大肠杆菌中过表达沙眼衣原体血清型F的45 kDa蛋白（45K）的基因。推导的肽序列与大肠杆菌延伸因子Tu（EF-Tu）的比对显示69％的同一性。 45K被衣原体属特异性单克隆抗体GP-45识别，并与针对大肠杆菌EF-Tu的单特异性多克隆抗体发生交叉反应。纯化的重组45K具有结合GDP的能力，并且在存在大肠杆菌延伸因子Ts（EF-Ts）的情况下增强了结合。 GDP结合被单克隆抗体GP-45特异性抑制。这些数据表明45K是衣原体EF-Tu，并且与大肠杆菌EF-Ts蛋白形成功能复合物。

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《Journal of bacteriology》 |1994年第4期|共4页
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Y X Zhang; Y Shi; M Zhou; G A Petsko;
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6. Cloning sequencing and expression in Escherichia coli of the gene encoding a 45-kilodalton protein elongation factor Tu from Chlamydia trachomatis serovar F. [O] . Y X Zhang, Y Shi, M Zhou, 1994

机译：沙眼衣原体沙眼衣原体F编码45千达尔顿蛋白延伸因子Tu的基因的克隆测序和在大肠杆菌中的表达。
7. Cloning, sequencing, and expression in Escherichia coli of the gene encoding a 45-kilodalton protein, elongation factor Tu, from Chlamydia trachomatis serovar F. [O] . Zhang, Y X, Shi, Y, Zhou, M, 1994

机译：沙眼衣原体沙眼衣原体F编码45千达尔顿蛋白，延伸因子Tu的基因的克隆，测序和在大肠杆菌中的表达。
8. Cloning and Expression in 'Escherichia coli' of Pseudomonas Strain LB400 Genes Encoding Polychlorinated Biphenyl Degradation [R] . Mondello, F. J. 1989

机译：多氯联苯降解的假单胞菌LB400基因'大肠杆菌'的克隆与表达

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Cloning, sequencing, and expression in Escherichia coli of the gene encoding a 45-kilodalton protein, elongation factor Tu, from Chlamydia trachomatis serovar F.

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