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首页> 外文期刊>Journal of bacteriology >Mannitol, a novel bacterial compatible solute in Pseudomonas putida S12.
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Mannitol, a novel bacterial compatible solute in Pseudomonas putida S12.

机译：甘露醇，恶臭假单胞菌S12中的一种新型细菌相容性溶质。

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The aim of this study was to identify the compatible solutes accumulated by Pseudomonas putida S12 subjected to osmotic stress. In response to reduced water activity, P. putida S12 accumulated Nalpha-acetylglutaminylglutamine amide (NAGGN) simultaneously with a novel compatible solute identified as mannitol (using 13C- and 1H-nuclear magnetic resonance, liquid chromatography-mass spectroscopy and high-performance liquid chromatography methods) to maximum concentrations of 74 and 258 micromol g (dry weight) of cells(-1), respectively. The intracellular amounts of each solute varied with both the type and amount of osmolyte applied to induce osmotic stress in the medium. Both solutes were synthesized de novo. Addition of betaine to the medium resulted in accumulation of this compound and depletion of both NAGGN and mannitol. Mannitol and NAGGN were accumulated when sucrose instead of salts was used to reduce the medium water activity. Furthermore, both compatible solutes were accumulated when glucose was substituted by other carbon sources. However, the intracellular quantities of mannitol decreased when fructose, succinate, or lactate were applied as a carbon source. Mannitol was also raised to high intracellular concentrations by other salt-stressed Pseudomonas putida strains. This is the first study demonstrating a principal role for the de novo-synthesized polyol mannitol in osmoadaptation of a heterotrophic eubacterium.
机译：这项研究的目的是确定在渗透胁迫下恶臭假单胞菌S12积累的相容性溶质。为响应减少的水活度，恶臭假单胞菌S12同时积聚Nalpha-乙酰谷氨酰胺基谷氨酰胺（NAGGN）和一种被鉴定为甘露醇的新型相容溶质（使用13C和1H核磁共振，液相色谱-质谱和高效液相色谱法方法）的最大浓度分别为74和258微摩尔g（干重）的细胞（-1）。每种溶质的细胞内量随所施加的在培养基中诱导渗透压的渗透液的类型和量而变化。两种溶质都是从头合成的。向培养基中添加甜菜碱会导致该化合物积聚，同时NAGGN和甘露醇均会耗尽。当使用蔗糖代替盐降低培养基中的水分活度时，甘露醇和NAGGN会积累。此外，当葡萄糖被其他碳源取代时，两种相容性溶质都会积累。然而，当果糖，琥珀酸盐或乳酸盐用作碳源时，甘露醇的细胞内量减少。其他盐胁迫的假单胞菌恶臭假单胞菌菌株也将甘露醇升高至高细胞内浓度。这是首次证明从头合成的多元醇甘露醇在异养真细菌的渗透适应中的主要作用。

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