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首页> 外文期刊>Journal of bacteriology >Proposed pathway for biosynthesis of the S-layer glycoprotein of Bacillus alvei.
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Proposed pathway for biosynthesis of the S-layer glycoprotein of Bacillus alvei.

机译:芽孢杆菌S层糖蛋白生物合成的拟议途径。

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摘要

The outer surface of the murein sacculus of the eubacterium Bacillus alvei is covered by a surface layer (S-layer) glycoprotein. The glycan chain of this S-layer glycoprotein consists of trisaccharide repeating units with ManNAc, Gal, and Glc as constituents. From cell extracts of B. alvei, nucleotide-activated derivatives of ManNAc, Gal, Glc, and GlcNAc were isolated. Furthermore, GDP- and dolichyl-activated oligosaccharides were obtained. On the basis of the isolated putative glycoprotein precursors, a pathway for the biosynthesis of the oligosaccharide chain is proposed.
机译:真细菌芽孢杆菌的壁膜囊泡的外表面被表层(S层)糖蛋白覆盖。该S层糖蛋白的聚糖链由三糖重复单元组成,其中ManNAc,Gal和Glc为组成部分。从B. alvei的细胞提取物中,分离出ManNAc,Gal,Glc和GlcNAc的核苷酸激活衍生物。此外,获得了GDP和二硫酰基活化的寡糖。在分离出的假定糖蛋白前体的基础上,提出了寡糖链生物合成的途径。

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