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首页> 外文期刊>Journal of bacteriology >Responses to nutrient starvation in Pseudomonas putida KT2442: two-dimensional electrophoretic analysis of starvation- and stress-induced proteins.
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Responses to nutrient starvation in Pseudomonas putida KT2442: two-dimensional electrophoretic analysis of starvation- and stress-induced proteins.

机译：恶臭假单胞菌KT2442对营养饥饿的反应：饥饿和应激诱导的蛋白质的二维电泳分析。

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摘要

The responses of Pseudomonas putida KT2442 to various forms of nutrient starvation and stress conditions were examined by two-dimensional polyacrylamide electrophoresis. Carbon deprivation resulted in a temporal expression of two classes of starvation-induced proteins: one class was transiently expressed during the initial phase of starvation, and the second class was expressed throughout the entire starvation period. Proteins of the second class could be further subdivided into proteins induced specifically under conditions of carbon starvation, proteins also induced by conditions of stress created by elevated temperature and osmolarity, and finally proteins that were also induced by conditions of nitrogen as well as phosphate starvation. Addition of glucose to a carbon-starved culture led to initiation of a recovery phase. During this phase, repression of starvation-induced proteins as well as induction of a new class of transiently expressed proteins, referred to as maturation proteins, took place.
机译：通过二维聚丙烯酰胺电泳检查恶臭假单胞菌KT2442对各种形式的营养饥饿和胁迫条件的响应。碳剥夺导致饥饿诱导的两类蛋白质的时间表达：一类在饥饿的初始阶段短暂表达，第二类在整个饥饿时期表达。第二类蛋白质可以进一步细分为在碳饥饿条件下特异性诱导的蛋白质，也由高温和渗透压产生的压力条件诱导的蛋白质，最后也由氮和磷酸盐饥饿条件诱导的蛋白质。将葡萄糖添加到碳缺乏的培养物中导致恢复阶段的开始。在这一阶段，进行了饥饿诱导的蛋白的抑制以及一类新的瞬时表达蛋白的诱导，该蛋白被称为成熟蛋白。

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