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首页> 外文期刊>Journal of bacteriology >Mutations in the bvgA gene of Bordetella pertussis that differentially affect regulation of virulence determinants.
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Mutations in the bvgA gene of Bordetella pertussis that differentially affect regulation of virulence determinants.

机译：百日咳博德特氏菌bvgA基因突变，差异影响毒力决定因素的调节。

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摘要

By using chemical mutagenesis and genetic mapping, a search was undertaken for previously undescribed genes which may be involved in different regulatory mechanisms governing different virulence factors of Bordetella pertussis. Previous studies have shown that the fha locus encoding filamentous hemagglutinin is regulated directly by the bvgAS two component system, while regulation of ptx encoding pertussis toxin is less direct or occurs by a different mechanism. With a strain containing gene fusions to each of these regulated loci, screening was done for mutations which were defective for ptx expression but maintained normal or nearly normal levels of fha expression. Two mutations which had such a phenotype and were also deficient in adenylate cyclase toxin/hemolysin expression were found and characterized more fully. Both were found to affect residues in the C-terminal portion of the BvgA response regulator protein, a domain which shares sequence similarity with a family of regulatory proteins including FixJ, UhpA, MalT, RcsA, RcsB, and LuxR. The residues affected are within a region which, by extension from studies on the LuxR protein, may be involved in transcriptional activation.
机译：通过使用化学诱变和遗传作图，对以前未描述的基因进行了搜索，这些基因可能参与了百日咳博德特氏菌不同毒力因子的不同调控机制。先前的研究表明，编码丝状血凝素的fha基因座直接由bvgAS两组分系统调节，而编码百日咳毒素的ptx调节则不太直接或由不同的机制发生。用含有与这些受调控基因座的每一个基因融合的菌株，进行对ptx表达有缺陷但保持正常或接近正常水平的fha表达的突变的筛选。发现了两个具有这种表型并且也缺乏腺苷酸环化酶毒素/溶血素表达的突变，并对其进行了更充分的表征。发现两者都影响BvgA反应调节蛋白C端部分的残基，该结构域与一系列调节蛋白（包括FixJ，UhpA，MalT，RcsA，RcsB和LuxR）共享序列相似性。受影响的残基在一个区域内，通过对LuxR蛋白的研究扩展，该区域可能与转录激活有关。

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