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首页> 外文期刊>Journal of bacteriology >The XbaI-BlnI-CeuI genomic cleavage map of Salmonella paratyphi B.
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The XbaI-BlnI-CeuI genomic cleavage map of Salmonella paratyphi B.

机译：副伤寒沙门氏菌B的XbaI-BlnI-CeuI基因组切割图。

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The genomic cleavage map of Salmonella paratyphi B was determined through digestion with endonucleases and separation of the fragments by pulsed-field gel electrophoresis. The chromosome has 19 XbaI sites, 10 BlnI sites, and 7 CeuI sites. The fragments were arranged in order through excision of fragments from the gel, redigestion with a second enzyme, end labelling with 32P, and reelectrophoresis. Tn10 transposons inserted in 61 different genes of S. typhimurium LT2 were transduced by use of bacteriophage P22 into S. paratyphi B. The locations of Tn10 insertions on the chromosome of S. paratyphi B were determined by use of XbaI and BlnI sites in Tn10, revealing the positions of genes with Tn10 insertions in S. paratyphi B. All seven CeuI sites (in rrl genes for 23S rRNA) and most of the XbaI and BlnI sites in rrn genes for Glt-tRNA are conserved, but only about half of the XbaI and BlnI sites outside rrn genes are conserved. Gene order is identical in the 68 genes that we could compare between S. paratyphi B and S. typhimurium LT2, and the lengths of intervals between the genes are often the same, but there are several instances of differences in interval lengths, indicating that insertions or deletions of DNA have occurred during the evolutionary divergence of these bacteria.
机译：通过用内切核酸酶消化并通过脉冲场凝胶电泳分离片段来确定副伤寒沙门氏菌B的基因组切割图。该染色体具有19个XbaI位点，10个BlnI位点和7个CeuI位点。通过从凝胶上切下片段，用第二种酶重新分配，用32P末端标记和重新电泳，依次排列片段。使用噬菌体P22将Sn鼠伤寒沙门氏菌LT2的61个不同基因中插入的Tn10转座子转导至副伤寒沙门氏菌B中。揭示了在副伤寒沙门氏菌中有Tn10插入的基因的位置。所有七个CeuI位点（在23S rRNA的rrl基因中）以及Glt-tRNA的rrn基因中的大多数XbaI和BlnI位点均被保留，但仅约一半rrn基因外的XbaI和BlnI位点是保守的。我们可以在副伤寒沙门氏菌B和鼠伤寒沙门氏菌LT2之间比较的68个基因中，基因顺序是相同的，并且基因之间的间隔长度通常是相同的，但是在间隔长度上存在几种不同的情况，这表明插入这些细菌的进化趋异过程中发生了DNA缺失或DNA缺失。

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