Chi-dependent formation of linear plasmid DNA in exonuclease-deficient recBCD+ strains of Escherichia coli.

M M Zaman; T C Boles

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Chi-dependent formation of linear plasmid DNA in exonuclease-deficient recBCD+ strains of Escherichia coli.

机译：大肠杆菌中核酸外切酶缺陷型recBCD +菌株中Chi依赖型线性质粒DNA的形成。

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Escherichia coli strains carrying mutations in sbcB (exonuclease I) or xthA (exonuclease III) accumulate high-molecular-weight linear plasmid concatemers when transformed with plasmids containing the chi sequence, 5'-GCTGGTGG-3'. Chi-dependent formation of high-molecular-weight plasmid DNA is dependent on recA and recF functions. In addition, chi stimulation occurs only in cis. Our data are consistent with models in which RecA and RecF proteins bind to and protect the DNA ends produced by RecBCD-chi interaction.

机译：当用含有chi序列5'-GCTGGTGG-3'的质粒转化时，携带sbcB（核酸外切酶I）或xthA（核酸外切酶III）突变的大肠杆菌菌株会积聚高分子量线性质粒串联体。高分子量质粒DNA的Chi依赖性形成取决于recA和recF功能。另外，chi刺激仅在顺式中发生。我们的数据与其中RecA和RecF蛋白结合并保护RecBCD-chi相互作用产生的DNA末端的模型一致。

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《Journal of bacteriology》 |1994年第16期|共8页
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M M Zaman; T C Boles;
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中图分类微生物学;
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6. Chi-dependent formation of linear plasmid DNA in exonuclease-deficient recBCD+ strains of Escherichia coli. [O] . M M Zaman, T C Boles 1994

机译：大肠杆菌中核酸外切酶缺陷型recBCD +菌株中Chi依赖型线性质粒DNA的形成。
7. High-molecular-weight linear multimer formation by single-stranded DNA plasmids in Escherichia coli. [O] . Dabert, P, Ehrlich, S D, Gruss, A 1992

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Chi-dependent formation of linear plasmid DNA in exonuclease-deficient recBCD+ strains of Escherichia coli.

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