...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >Pseudomonas aeruginosa 142 uses a three-component ortho-halobenzoate 1,2-dioxygenase for metabolism of 2,4-dichloro- and 2-chlorobenzoate.
【24h】

Pseudomonas aeruginosa 142 uses a three-component ortho-halobenzoate 1,2-dioxygenase for metabolism of 2,4-dichloro- and 2-chlorobenzoate.

机译：铜绿假单胞菌142使用三组分原卤代苯甲酸酯1,2-二加氧酶来代谢2,4-二氯-和2-氯苯甲酸酯。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cell extracts of Pseudomonas aeruginosa 142, which was previously isolated from a polychlorinated biphenyl-degrading consortium, were shown to degrade 2,4-dichlorobenzoate, 2-chlorobenzoate, and a variety of other substituted ortho-halobenzoates by a reaction that requires oxygen, NADH, Fe(II), and flavin adenine dinucleotide. By using extracts that were chromatographically depleted of chlorocatechol and catechol 1,2-dioxygenase activities, products of the initial reaction with 2,4- or 2,5-dichlorobenzoate and 2-chlorobenzoate were identified by mass spectrometry as 4-chlorocatechol and catechol. In contrast to the well-characterized benzoate dioxygenases or the recently described 2-halobenzoate 1,2-dioxygenase from P. cepacia 2CBS (S. Fetzner, R. Müller, and F. Lingens, J. Bacteriol. 174:279-290, 1992) that possess two protein components, the P. aeruginosa enzyme was resolved by ion-exchange chromatography into three components, each of which is required for activity. To verify the distinct nature of this enzyme, we purified, characterized, and identified one component as a ferredoxin (M(r), approximately 13,000) containing a single [2Fe-2S] Rieske-type cluster (electron paramagnetic resonance spectroscopic values of gx = 1.82, gy = 1.905, and gz = 2.02 in the reduced state) that is related in sequence to ferredoxins found in the naphthalene and biphenyl three-component dioxygenase systems. By analogy to these enzymes, we propose that the P. aeruginosa ferredoxin serves as an electron carrier between an NADH-dependent ferredoxin reductase and the terminal component of the ortho-halobenzoate 1,2-dioxygenase. The broad specificity and high regiospecificity of the enzyme make it a promising candidate for use in the degradation of mixtures of chlorobenzoates.
机译：铜绿假单胞菌142的细胞提取物先前已从多氯联苯降解财团中分离出来，显示其可通过需要氧气的反应（NADH）降解2,4-二氯苯甲酸酯，2-氯苯甲酸酯和多种其他取代的邻卤代苯甲酸酯。 ，Fe（II）和黄素腺嘌呤二核苷酸。通过使用色谱分离的氯邻苯二酚和邻苯二酚1,2-二加氧酶活性的提取物，通过质谱鉴定与2,4-或2,5-二氯苯甲酸酯和2-氯苯甲酸酯的初始反应产物为4-氯邻苯二酚和邻苯二酚。与表征良好的苯甲酸酯双加氧酶或最近描述的来自洋葱伯克霍尔德菌2CBS的2-卤代苯甲酸酯1,2-二加氧酶（S.Fetzner，R.Müller，and F.Lingens，J.Bacteriol.174：279-290， 1992年）具有两个蛋白质成分，铜绿假单胞菌酶通过离子交换色谱分解为三个成分，每个成分都是活性所必需的。为了验证该酶的独特性质，我们纯化，鉴定和鉴定了一种成分为铁氧还蛋白（铁（M（r），约13,000）），其中含有单个[2Fe-2S] Rieske型簇（gx的电子顺磁共振光谱值）在还原状态下，其与苯并三联体双组分双加氧酶系统中的铁氧还蛋白顺序相关。Gy= 1.82，gy = 1.905，gz = 2.02。通过类似于这些酶，我们建议铜绿假单胞菌铁氧还蛋白充当NADH依赖性铁氧还蛋白还原酶与邻卤代苯甲酸酯1，2-二加氧酶的末端组分之间的电子载体。该酶的广泛特异性和高区域特异性使其成为用于降解氯苯甲酸酯混合物的有希望的候选者。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号