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首页> 外文期刊>Journal of bacteriology >Flagellar filament structure and cell motility of Salmonella typhimurium mutants lacking part of the outer domain of flagellin.
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Flagellar filament structure and cell motility of Salmonella typhimurium mutants lacking part of the outer domain of flagellin.

机译：缺乏鞭毛蛋白外部结构域的鼠伤寒沙门氏菌突变体的鞭毛细丝结构和细胞运动性。

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摘要

We have isolated spontaneous mutants of Salmonella typhimurium which can swim in the presence of antifilament antibodies. The molecular masses of flagellins isolated from these mutants were smaller than that (52 kDa) of wild-type flagellin. Two mutants which produced the smallest flagellins (42 and 41 kDa) were selected, and the domain structures of the flagellins were analyzed by trypsin digestion and then subjected to amino acid sequencing. The two flagellins have deletions at Ala-204 to Lys-292 and Thr-183 to Lys-279, respectively. These deleted parts belong to the outer domain (D3) of flagellin, which is believed to be at the surface of the filament. These mutant filaments aggregated side by side in the presence of salt, resulting in disordered motility.
机译：我们已经分离出鼠伤寒沙门氏菌的自发突变体，可以在存在抗丝抗体的情况下游泳。从这些突变体中分离出的鞭毛蛋白的分子量小于野生型鞭毛蛋白的分子量（52 kDa）。选择产生最小鞭毛蛋白（42和41kDa）的两个突变体，并通过胰蛋白酶消化分析鞭毛蛋白的结构域结构，然后进行氨基酸测序。两种鞭毛蛋白分别在Ala-204至Lys-292和Thr-183至Lys-279具有缺失。这些缺失的部分属于鞭毛蛋白的外部结构域（D3），认为其位于细丝的表面。这些突变体细丝在盐的存在下并排聚集，导致运动性紊乱。

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