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首页> 外文期刊>Journal of bacteriology >Genomic fluidity of Bordetella pertussis assessed by a new method for chromosomal mapping.
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Genomic fluidity of Bordetella pertussis assessed by a new method for chromosomal mapping.

机译：通过一种新的染色体作图方法评估百日咳博德特氏菌的基因组流动性。

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The genomic organization of Bordetella pertussis strains has been examined by using a new method. This method does not depend on the prior determination of a restriction map of the bacterial chromosome but is based on the ability to measure directly the distance between two genes. This is accomplished through the integration at each gene of a suicide vector containing a cleavage site for the intron-encoded endonuclease I-SceI, which is not otherwise found in the chromosome. Integration is mediated by homologous recombination between the chromosomal and cloned plasmid copies of a gene of interest. Digestion with I-SceI gives rise to a fragment the size of which represents the distance between the two genes. Multiple pairwise determinations within a set of genes provide sufficient information to derive a map of the relative gene positions. Mapping a set of 11 to 13 genes for five strains of B. pertussis and one strain of B. parapertussis revealed extensive divergence of gene order between B. pertussis Tohama I, B. pertussis 18-323, and B. parapertussis ATCC 15311. Less extensive divergence of gene order was observed between B. pertussis Tohama I and B. pertussis Tohama III, BP165, and Wellcome 28, with most of the observed differences explainable by large inversions.
机译：百日咳博德特氏菌菌株的基因组结构已通过使用新方法进行了检查。该方法不依赖于事先确定细菌染色体限制性图谱，而是基于直接测量两个基因之间距离的能力。这是通过在自杀基因的每个基因上整合而完成的，该自杀载体包含内含子编码的核酸内切酶I-SceI的切割位点，而该切割位点在染色体中没有发现。整合由目的基因的染色体和克隆质粒拷贝之间的同源重组介导。用I-SceI消化产生一个片段，其大小代表两个基因之间的距离。一组基因中的多个成对确定可提供足够的信息来推导相对基因位置的图谱。对五个百日咳博德特氏菌和一个百日咳博德特氏菌菌株的一组11至13个基因进行定位，发现百日咳博德特氏菌Tohama I，百日咳博德特氏菌18-323和百日咳博德特氏菌ATCC 15311之间的基因顺序差异很大。在百日咳博德特氏菌Tohama I和百日咳博德特氏菌Tohama III，BP165和Wellcome 28之间观察到基因顺序的广泛差异，其中观察到的大多数差异可以通过大的倒位来解释。

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