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首页> 外文期刊>Journal of bacteriology >Identification of Campylobacter jejuniPromoter Sequences
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Identification of Campylobacter jejuniPromoter Sequences

机译:空肠弯曲菌启动子序列的鉴定

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摘要

A promoterless lacZ shuttle vector, which allowed screening of promoters by β-galactosidase activity inCampylobacter jejuni and Escherichia coli, was developed. Chromosomal DNA fragments from C. jejuniwere cloned into this vector; 125 of 1,824 clones displayed promoter activity in C. jejuni. Eleven clones with strong promoter activity in C. jejuni were further characterized. Their nucleotide sequences were determined, and the transcriptional start sites of the putative promoters in C. jejuni were determined by primer extension. Only 6 of these 11 promoters were functional in E. coli. The 11 newly characterized and 10 previously characterized C. jejuni promoters were used to establish a consensus sequence for C. jejuni promoters. The 21 promoters were found to be very similar. They contain three conserved regions, located approximately 10, 16, and 35 bp upstream of the transcriptional start point. The ?10 region resembles that of a typical ?70 E. colipromoter, but the ?35 region is completely different. In addition a ?16 region typical for gram-positive bacteria was identified.
机译:开发了无启动子的 lacZ 穿梭载体,该载体可通过空肠弯曲杆菌和大肠杆菌中的β-半乳糖苷酶活性筛选启动子。来自 C的染色体DNA片段。空肠被克隆到该载体中; 1,824个克隆中的125个在 C中表现出启动子活性。空肠。在 C中具有强启动子活性的11个克隆。空肠的进一步特征。确定了它们的核苷酸序列,并确定了 C中推定的启动子的转录起始位点。通过引物延伸确定空肠。这11个启动子中只有6个在 E中起作用。大肠杆菌。 11个新表征的和10个先前表征的 C。空肠启动子被用于建立 C的共有序列。空肠启动子。发现21个启动子非常相似。它们包含三个保守区,位于转录起点上游约10、16和35 bp。 ?10区域类似于典型的 sup> 70 E区域。大肠杆菌启动子,但?35区域完全不同。另外,鉴定出革兰氏阳性细菌典型的α16区。

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