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首页> 外文期刊>Journal of bacteriology >Stimulation of Transcription by Mutations Affecting Conserved Regions of RNA Polymerase II
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Stimulation of Transcription by Mutations Affecting Conserved Regions of RNA Polymerase II

机译：通过影响RNA聚合酶II保守区的突变刺激转录。

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Mutations that increase the low-level transcription of theSaccharomyces cerevisiae HIS4 gene, which results from deletion of the genes encoding transcription factors BAS1, BAS2, and GCN4, were isolated previously in SIT1 (also known asRPO21, RPB1, and SUA8), the gene encoding the largest subunit of RNA polymerase II (RNAPII). Here we show that sit1 substitutions cluster in two conserved regions of the enzyme which form part of the active site. Sixsit1 mutations, affect region F, a region that is involved in transcriptional elongation and in resistance to α-aminatin. Foursit1 substitutions lie in another region involved in transcriptional elongation, region D, which binds Mg2+ ions essential for RNA catalysis. One region D substitution is lethal unless suppressed by a substitution in region G and interacts genetically withPPR2, the gene encoding transcription elongation factor IIS. Some sit1 substitutions affect the selection of transcriptional start sites at the CYC1 promoter in a manner reminiscent of that of sua8 (sua stands for suppression of upstream ATG) mutations. Together with previous findings which indicate that regions D and G are in close proximity to the 3′ end of the nascent transcript and that region F is involved in the translocation process, our results suggest that transcriptional activation by the sit1 mutations results from alteration of the RNAPII active center.
机译：先前在 SIT1 基因低水平转录的突变，该突变是由于编码转录因子BAS1，BAS2和GCN4的基因缺失所致。 >（也称为 RPO21 ， RPB1 和 SUA8 ），该基因编码RNA聚合酶II（RNAPII）的最大亚基。在这里，我们显示 sit1 替换簇聚在酶的两个保守区域中，形成了活性位点。六个 sit1 突变影响区域F，该区域涉及转录延伸和对α-氨基甜菜碱的抗性。四个 sit1 取代位于另一个参与转录延伸的区域，即区域D，该区域与RNA催化必不可少的Mg 2 + 离子结合。一个D区取代是致命的，除非被G区取代所抑制，并且与编码转录延伸因子IIS的基因 PPR2 发生相互作用。 sit1 的某些替换会影响 CYC1 启动子上转录起始位点的选择，其方式类似于 sua8 （ sua 代表抑制上游ATG）突变。连同先前的发现表明区域D和G紧密靠近新生转录本的3'末端，并且区域F参与了易位过程，我们的结果表明 sit1 突变是由RNAPII活性中心的改变引起的。

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