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首页> 外文期刊>Journal of bacteriology >Transposition of IS2 into the hemB gene of Escherichia coli K-12.
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Transposition of IS2 into the hemB gene of Escherichia coli K-12.

机译:IS2易位到大肠杆菌K-12的hemB基因中。

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摘要

Genetic studies of the hemB gene in Escherichia coli have resulted in the recovery of both stable and unstable mutant strains. The stable strains have been shown to result from large deletions. This study demonstrates that unstable strains result from the insertion of transposable element IS2 primarily into the 5' region of the structural gene; the instability results from precise excision of the element, producing strains with both high and low frequencies of reversion. This first report of IS2 insertion into hemB suggests that this gene may be a preferred target for insertion of this transposable element.
机译:大肠杆菌中hemB基因的遗传研究已导致稳定和不稳定突变菌株的恢复。已显示稳定的菌株是由大的缺失产生的。这项研究表明,不稳定的菌株是由转座因子IS2主要插入结构基因的5'区域引起的。不稳定性是由于元件的精确切除所导致,产生了回复频率高低的应变。 IS2插入hemB的第一个报道表明,该基因可能是该转座因子插入的首选靶标。

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